The Effects of <i>Eunhoebanchong-san</i> on the Caerulein-induced Acute Pancreatitis in the Rat

Lee, Hyung-ho; Kim, In-soo; Shin, Min-gu; Lee, Young-su

The Journal of Internal Korean Medicine > Volume 34(3); 2013 > Article

Article

The Journal of Internal Korean Medicine 2013;34(3): 298-311.

Caerulein으로 유발된 흰쥐의 급성 췌장염에 대한 銀茴蟠葱散의 효과

이형호, 김인수, 신민구, 이영수

동신대학교 한의과대학 내과학교실

The Effects of Eunhoebanchong-san on the Caerulein-induced Acute Pancreatitis in the Rat

Hyung-ho Lee, In-soo Kim, Min-gu Shin, Young-su Lee

Dept. of Internal Medicine, College of Oriental Medicine, Dong-Shin University

Correspondence

Young-su Lee ,Tel: 062-350-7203, Fax: 062-350-7141, Email: hefari92@hanmail.net

Published online: September 30, 2013.

ABSTRACT

Objectives:
In this study, we aimed to investigate the effect of Eunhoebanchong-san (EBS) on caerulein-induced acute pancreatitis (AP) by detecting oxidative stress markers and performing histopathological examination.

Methods :
Thirty adult male Sprague-Dawley rats were divided into five groups as follows: normal (NOR), caerulein-induced (CON), caerulein+EBS (130 mg/kg, EA), caerulein+EBS (260 mg/kg, EB) and caerulein+EBS (520 mg/kg, EC) groups. Pancreatic tissues of rats from all groups were removed for apoptosis, and light, and electron microscopic examination. Blood of rats from all groups was obtained for oxidative stress markers and pathological examination. Pancreatic oxidative stress markers were evaluated by the measurements of serum amylase, and interleukin-6 (IL-6) levels were determined spectrophotometrically.

Results:
The ratio of pancreas/body weight increased significantly in the CON compared with the NOR, but decreased significantly in the EA, the EB, the EC groups compared with the CON. Caerulein administration resulted in a significant increase in amylase, but EBS reduced the levels of these enzymes. Interleukin-6 (IL-6) levels increased significantly in CON compared with NOR, but reduced in EA, EB, and EC group at 24 hrs. In the observations of optical microscopy and electron microscopy, the experimental groups showed significant decreases in pancreatic tissue inflammation, edema, vacuolization, necrosis compared to the control group. Altogether, this indicates EBS is potentially capable of limiting pancreatic damage produced during AP by restoring the fine structure of acinar cells and tissue.

Conclusions:
We concluded that EBS may have beneficial effects in the treatment of caerulein-induced AP.

Key words: Eunhoebanchong-san, Caerulein, acute pancreatitis, Interleukin-6 (IL-6), acinar cell