| 加減大黃芒硝湯 및 그 구성약재가 Cerulein으로 유도된 생쥐의 急性 膵臟炎에 미치는 影響 |
| 유주연, 유근정, 신용진, 이언정 |
| 원광대학교 한의과대학 내과학교실 |
| Effects of an Herbal Medicine, Gagam-daewhang mangcho-tang, and its Components
on Cerulein-induced Acute Pancreatitis in Mice |
| Ju-yeon Yu, Keun-jeong Yu, Yong-jeen Shin, Un-jung Lee |
| Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University |
|
|
|
Received: April 24, 2015, Accepted: June 24, 2015, Published online: June 30, 2015. |
|
|
|
| ABSTRACT |
Objectives:
This study investigated the effects of Gagam-daewhang mangcho-tang (GDM) and its components on cerulein-induced acute pancreatitis (AP) in mice.
Methods:
The AP mouse model was induced by intra-peritoneal injection of cerulein (50 μg/kg) at hourly intervals for 6 times. The experimental drug was administrated intraperitoneally 1 hour prior to the first injection of cerulein. Mice were sacrificed at 6 hours after the last injection of cerulein. Blood samples were taken to determine serum amylase level. The pancreas and lungs were rapidly removed for histochemical examination and myeloperoxidase (MPO) assays.
Results:
Administration of modified GDM significantly reduced the ratio of pancreas/body weight, level of serum amylase, neutrophil infiltration, and histological damage of the pancreas and lung. In a test of the components of GDM, the Salvia miltiorrhiza (SM) group showed a significant suppression of the severity of AP. In an experiment testing the concentration of SM, the 150 mg/kg SM group showed significant attenuation of the severity of AP.
Conclusions:
Modified GDM and a SM water extract could attenuate AP and AP-associated lung injury via suppression of digestive enzyme secretion and MPO activity. |
| Key words:
acute pancreatitis, Daewhang mangcho-tang, Daio bosho-to |
|
PDF Links
Full text via DOI 
Download Citation 
