| Antioxidative Effects of Lycium chinense Miller on Cisplatin-induced
Nephrotoxicity in Rats |
| Yu-sun Jung1, Chan-hum Park2, Hyeon-cheol Shin1 |
1Dept. of Internal Medicine of Korean Medicine, College of Korean Medicine, Dae-gu Haany University
2Dept. of Herbal Medicine, College of Korean Medicine, Dae-gu Haany University |
| Correspondence |
Hyeon-cheol Shin ,Tel: 054-281-0055, Fax: 054-281-7464, Email: ungaeshin@naver.com
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Published online: March 30, 2014. |
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| ABSTRACT |
Objectives:
Cisplatin is a widely used cancer therapy drug. However, nephrotoxicity resulting in increased oxidative stress is a major side effect of cisplatin chemotherapy, thereby limiting its chemotherapeutic use. Lycium chinense Miller (LCM) has been used as a traditional herbal medicine in various febrile and inflammatory diseases such as night sweat, cough, nosebleed, bronchitis, pulmonary tuberculosis, etc. In this study we investigated the protective and antioxidative potential of LCM against cisplatin-induced nephrotoxicity in rats.
Methods :
Twenty-four 8-week-old male Wistar rats were divided into four groups: normal untreated; cisplatin treatment only; LCM 10 mg/kg plus cisplatin treatment; and LCM 30 mg/kg plus cisplatin treatment. Twenty-four hours after the last cisplatin injection, all the rats were sacrificed, and serological changes were evaluated. The levels of NF-κB activity and NOX-4, p47phox, p22phox, COX-2, iNOS, SOD, catalase expressions were analyzed in Western blot analysis.
Results:
Cisplatin injection caused an increase in the BUN level, which is a reliable indicator of renal toxicity. The levels of BUN, renal ROS, and renal TBARS were significantly reduced in the LCM groups compared with the cisplatin-only groups. The levels of p47phox and p22phox, which are NADPH oxidase subunits, were increased in the cisplatin-only groups, whereas they were decreased in the LCM groups. The levels of renal NF-κB activity and COX-2, iNOS expressions were increased significantly in the cisplatin-only groups compared with the normal groups, whereas they were decreased in the LCM groups. Compared with the cisplatin-only groups, renal GSH and GSH/GSSG increased in the LCM groups. Also, the administration of LCM increased levels of SOD and catalase as compared with the cisplatin-only groups.
Conclusions:
These results suggest that LCM protects cisplatin-induced nephrotoxicity via a mechanism that may involves the inhibition of oxidative stress by the activation of antioxidants. |
| Key words:
Lycium chinense Miller, cisplatin, nephrotoxicity, NF-κB, GSH |
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