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The Journal of Internal Korean Medicine 2004;25(2): 268-276. |
| 補骨脂의 Peroxynitrite 제거 기전 |
| 정지천1, 장용석1, 민건우2 |
1동국대학교 한의과대학 내과학교실
2자인한방병원 내과 |
| Peroxynitrite Scavenging Mechanism of Psoralea corylifolia |
| Ji-Cheon Jeong1, Yong-Suk Jang1, Gun-Woo Min2 |
1Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University
2Dept. of Internal Medicine, Jain Oriental Hospital |
| Correspondence |
Ji-Cheon Jeong ,Tel: 02)3416-9731, Fax: 02)3444-9171, Email: jjcjh@hitel.net
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Received: April 23, 2004, Accepted: May 13, 2004, Published online: June 30, 2004. |
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| ABSTRACT |
Objective :
Peroxynitrite (ONOO-), formed from the reaction of O2- and NO, is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate scavenging activities of ONOO- and its precursors, NO and O2- and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123. .
Methods :
Psoralea corylifolia was crushed. The crushed Psoralea corylifolia was extracted 3 times, each time with 3 volumes of methyl alcohol at 60℃ for 24 h. The extract was filtered and evaporated under reduced pressured using a rotary evaporator to yield 16 g. This was done to investigate scavenging activities of ONOO-, NO, O2- and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123.
Results:
After Psoralea corylifolia was added authentic ONOO-, •O2- and NO was markedly scavenged. Also, ONOOinduced by O2 - and NO (these derived from SIN-1) was inhibited. The data showed a decrease in ONOO- mediated nitration of tyrosine through electron donation after Psoralea corylifolia was added. Data showed a dose-dependent correlation with inhibition of nitration of bovine serum albumin induced by ONOO-. Furtheremore, LPS-induced ROS and RNS generation was blocked.
Conclusions:
These results suggest potential for use of Psoralea corylifolia as an effective ONOO- scavenger to counter the aging process and age-related diseases. |
| Key words:
Peroxynitrite, Scavenging Mechanism, Psoralea corylifolia |
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