Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81 Warning: fopen(/home/virtual/jikm/journal/upload/ip_log/ip_log_2022-06.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83 Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Peroxynitrite Scavenging Mechanism of Psoralea corylifolia
| Home | E-Submission | Sitemap | Editorial Office |  
top_img
The Journal of Internal Korean Medicine > Volume 25(2); 2004 > Article
The Journal of Internal Korean Medicine 2004;25(2): 268-276.
補骨脂의 Peroxynitrite 제거 기전
정지천1, 장용석1, 민건우2
1동국대학교 한의과대학 내과학교실
2자인한방병원 내과
Peroxynitrite Scavenging Mechanism of Psoralea corylifolia
Ji-Cheon Jeong1, Yong-Suk Jang1, Gun-Woo Min2
1Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University
2Dept. of Internal Medicine, Jain Oriental Hospital
Correspondence  Ji-Cheon Jeong ,Tel: 02)3416-9731, Fax: 02)3444-9171, Email: jjcjh@hitel.net
Received: April 23, 2004,   Accepted: May 13, 2004,   Published online: June 30, 2004.
ABSTRACT
Objective :
Peroxynitrite (ONOO-), formed from the reaction of O2- and NO, is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate scavenging activities of ONOO- and its precursors, NO and O2- and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123. .

Methods :
Psoralea corylifolia was crushed. The crushed Psoralea corylifolia was extracted 3 times, each time with 3 volumes of methyl alcohol at 60℃ for 24 h. The extract was filtered and evaporated under reduced pressured using a rotary evaporator to yield 16 g. This was done to investigate scavenging activities of ONOO-, NO, O2- and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123.

Results:
After Psoralea corylifolia was added authentic ONOO-, •O2- and NO was markedly scavenged. Also, ONOOinduced by O2 - and NO (these derived from SIN-1) was inhibited. The data showed a decrease in ONOO- mediated nitration of tyrosine through electron donation after Psoralea corylifolia was added. Data showed a dose-dependent correlation with inhibition of nitration of bovine serum albumin induced by ONOO-. Furtheremore, LPS-induced ROS and RNS generation was blocked.

Conclusions:
These results suggest potential for use of Psoralea corylifolia as an effective ONOO- scavenger to counter the aging process and age-related diseases.
Key words: Peroxynitrite, Scavenging Mechanism, Psoralea corylifolia
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
  E-Mail
Share:      
METRICS
617
View
6
Download
Related articles
Peroxynitrite Scavenging Activity of Samjunghwan  2006 March;27(1)
Peroxynitrite Scavenging Mechanism of Zingiberis Rhizoma  2003 March;24(1)
Editorial Office
Dongguk University Ilsan Oriental Hospital
27, Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326 Korea
TEL: +82-31-961-9046   FAX: +82-31-961-9049   E-mail: jikm.edit@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Society of Internal Korean Medicine.                 Developed in M2PI      |      Admin Login