Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81 Warning: fopen(/home/virtual/jikm/journal/upload/ip_log/ip_log_2022-06.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83 Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Peroxynitrite Scavenging Mechanism of Zingiberis Rhizoma
| Home | E-Submission | Sitemap | Editorial Office |  
The Journal of Internal Korean Medicine > Volume 24(1); 2003 > Article
The Journal of Internal Korean Medicine 2003;24(1): 75-83.
生薑의 Peroxynitrite 제거 기전
Peroxynitrite Scavenging Mechanism of Zingiberis Rhizoma
Sang-Guk Shin, Ji-Cheon Jeong
Department of Internal Medicine, College of Oriental Medicine, Dongguk University
Correspondence  Ji-Cheon Jeong ,Tel: 02-3416-9731, Fax: 02-3444-9171, Email: jjcjh@hitel.net
Received: December 6, 2002,   Accepted: February 11, 2003,   Published online: March 30, 2003.
Peroxynitrite (ONOO-), formed from the reaction of O2- and NO, is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been involved in the aging process and age-related diseases such as Alzheimer s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate scavenging activities of ONOO- and its precursors, NO and O2- and its scavenging mechanism of Zingiberis Rhizoma (ZR).

Methods :
To investigate scavenging activities of ONOO-, NO, O2- and its scavenging mechanism, we used fluorescent probes like DCFDA, DAF-2 and DHR 123. The ONOO- scavenging activity on ZR was assayed by measuring oxidized dihydrorhodamine 123 (DHR 123) by fluorometry. The scavenging efficacy was expressed as IC50, showing the concentration of each sample that is required to cause 50% inhibition of DHR 123 oxidation. In a separate study, the protective effect of ZR on ONOO--induced nitration of bovine serum albumin was investigated through immuno-assay with a monoclonal anti-nitryrosine antibody, and a horseradish peroxidase-conjugated anti-mouse secondary antibody from sheep.

ZR markedly scavenged authentic ONOO-, O2- and NO. It also inhibited ONOO- induced by O2- and NO which are derived from SIN-1. The data demonstrated that ZR led to decreased ONOO- mediated nitration of tyrosine through electron donation. It also inhibited the nitration of bovine serum albumin induced by ONOO- in a dose-dependent manner. Furtheremore, it blocked LPS-induced ROS and RNS generation.

These results suggest that ZR can be developed as an effective ONOO- scavenger for the prevention of aging process and age-related diseases.
Key words: Peroxynitrite, Scavenging Mechanism, Zingiberis Rhizoma, nitric oxide, superoxide anion
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
Related articles
Peroxynitrite Scavenging Activity of Samjunghwan  2006 March;27(1)
Peroxynitrite Scavenging Mechanism of Psoralea corylifolia  2004 June;25(2)
Editorial Office
Dongguk University Ilsan Oriental Hospital
27, Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326 Korea
TEL: +82-31-961-9046   FAX: +82-31-961-9049   E-mail: jikm.edit@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Society of Internal Korean Medicine.                 Developed in M2PI      |      Admin Login