The Effects of Soamgudamikgitang on the Immuno-modulation and Gap Junctional Intercellular Communication

Bang, Dae-Geon; Yoon, Sang-Hyub; Ryu, Bong-Ha; Ryu, Ki-Won

The Journal of Internal Korean Medicine > Volume 23(4); 2002 > Article

Original Article

The Journal of Internal Korean Medicine 2002;23(4): 579-586.

소암거담익기탕의 면역조절작용과 세포간 교통에 대한 연구

방대건, 윤상협, 류봉하, 류기원

경희대학교 한의과대학 비계내과학교실

The Effects of Soamgudamikgitang on the Immuno-modulation and Gap Junctional Intercellular Communication

Dae-Geon Bang, Sang-Hyub Yoon, Bong-Ha Ryu, Ki-Won Ryu

Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung Hee University

Correspondence

Dae-Geon Bang ,Tel: 02-958-9140, Fax: 02-958-9136, Email: bb3090@freechal.com

Received: September 3, 2002, Accepted: October 3, 2002, Published online: December 30, 2002.

ABSTRACT

Objective :
In order to observe the effects of SAKT on anticancer properties and GJIC, we have measured cytotoxicity on WB-F344, restoring effects of inhibited GJIC and Immuno-modulation effect of SAKT.

Method:
1. Cytotoxicity of SAKT on WB-F344 was measured by MTT assay. 2. WB-F344 was processed with H₂O₂ and SAKT 200㎍/㎖, 400㎍/㎖ , and 800㎍/㎖ respectively. Transfering pattern of lucifer yellow was observed by Scrape loading dye transfer (SL/DT) assay. 3. Experimental mice were divided to sarcoma-180 injection group (control), sarcoma-180 with SAKT 10mg injection group(SAKT 10), and sarcoma-180 with SAKT 50mg injection group(SAKT 50). After that, B cell, T cell, macrophase and NK-cell rate in the abdominal cavity were evaluated by FACS analysis.

Results:
1. Cytotoxicity of SAKT was low on WB-F344 cell lines. 2. SAKT of 200㎍/㎖ concentration was significantly effective in the restoration of GJIC(p<0.01). However, effects of SAKT both of 400㎍/㎖ and 800㎍/㎖ concentration were not significant. 3. In the SAKT 10 group, T cell(p<0.001), B cell(p<0.01) and macrophage (p<0.001) were significantly increased, but the increase of NK cell was not significant. In the SAKT 50 group, T cell, B cell, macrophase and NK cell were not significantly increased.

Conclusion:
According to the above results, It is assumed that SAKT is useful for the prevention of tumorigenesis and for cancer treatment.

Key words: SAKT(Soamgudamikgitang), Immuno-modulation, GJIC(Gap Junctional Intercellular Communication)