Effects of <i>Gamijinhae-tang</i> and <i>Socheongryong-tang-ga-seokgo</i> on PMA- induced
Production of Airway Mucin and Expression of Airway MUC5AC Gene

Byun, Jun-seop; Park, Yang-chun; Yang, Su-young; An, Joung-jo; Park, So-ae

The Journal of Internal Korean Medicine > Volume 29(3); 2008 > Article

Original Article

The Journal of Internal Korean Medicine 2008;29(3): 765-777.

加味鎭咳湯과 小靑龍湯加石膏가 PMA로 유발된 기도뮤신의 생성 및 MUC5AC gene 발현에 미치는 영향

변준섭, 박양춘, 양수영, 안정조, 박소애

대전대학교 한의과대학 내과학교실

Effects of Gamijinhae-tang and Socheongryong-tang-ga-seokgo on PMA- induced Production of Airway Mucin and Expression of Airway MUC5AC Gene

Jun-seop Byun, Yang-chun Park, Su-young Yang, Joung-jo An, So-ae Park

Dept. of Internal Medicine, College of Oriental Medicine, Daejeon University.

Correspondence

Yang-chun Park ,Tel: 043-229-3704, Fax: 043-253-8757, Email: omdpyc@dju.kr

Published online: September 30, 2008.

ABSTRACT

Objectives:
In this study, the author tried to examine whether Gamijinhae-tang and Socheongryong-tang-ga-seokgo significantly affect both PMA-induced mucin production and MUC5AC gene expression from airway epithelial cells.

Materials and Methods :
Confluent NCI-H292 cells were pretreated for 30 min in the presence of JHT and STS and treated with PMA (10ng/㎖), to assess the effects of the agents on PMA-induced mucin production by enzyme-linked immunosorbent assay (ELISA). Also, the effects of the agents on PMA-induced MUC5AC gene expression from the same cells were investigated. Possible cytotoxicities of the agent were assessed by examining the rate of survival and proliferation of NCI-H292 cells after treatment of agents during 48 hrs.

Results:
(1) JHT and STS did not show significant cytotoxicity to NCI-H292 cells. (2) JHT significantly decreased PMA-induced mucin production from NCI-H292 cells. However, STS did not affect mucin production. (3) JHT significantly inhibit the expression levels of PMA-induced MUC5AC gene in NCI-H292 cells. STS slightly decreased the expression levels of PMA-induced MUC5AC gene.

Conclusion:
These results suggest that JHT can not only affect the production of mucin but also affect the expression of the mucin gene, and this explains the traditional use of JHT in oriental medicine. The effects of JHT and STS with their components should be further investigated using animal experimental models that simulate pathophysiology of airway diseases through future studies.

Key words: airway mucin, MUC5AC gene Gamijinhae-tang, Socheongryong-tang-ga-seokgo