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The Journal of Internal Korean Medicine > Volume 29(1); 2008 > Article
The Journal of Internal Korean Medicine 2008;29(1): 243-257.
導痰湯이 C2C12세포주로부터 myostatin발현에 의한 심근에 미치는 영향
이유승1, 신유정1, 박종혁1, 김승모2, 백경민1, 박치상1
1대구한의대 심계내과학교실
2대구한의대 내과학교실
Effect of Differentiation for Mouse Myoblast C2C12 Cells against Myostatin expression from Dodamtang
You-seung Lee1, Yoo-jeong Shin1, Jong-hyuk Park1, Seung-mo Kim2, Kyung-min Paek1, Chi-sang Park1
1Dept. of Cardiovascular and Neurologic Disease, College of Oriental Medicine, Daegu-Haany University
2Dept. of Internal Medicine, College of Oriental Medicine, Daegu-Haany University
Correspondence  Chi-sang Park ,Tel: 053-770-2105, Email: 2nae2@hanmail.net
  Published online: March 30, 2008.
ABSTRACT
Myostatin, a negative regulator of myogenesis, is shown to function by controlling the proliferation of myoblasts. In this study we show that myostatin is an inhibitor of myoblast differentiation and that this inhibition is mediated through Smad 3. To determine MyoD expression by Dodamtang treatment, we compared the expression pattern of C2C12 mouse myoblasts that constitutively express myostatin with control cells. In vitro, increasing concentrations of Dodamtang reversibly prevented the myogenic blockage of myoblasts by myostatin expression. ELISA assay, Western and confocal analysis indicated that treatment of Dodamtang to the low serum culture media increased the levels of MyoD leading to the inhibition of myogenic differentiation by myostatin. The stable transfection of C2C12 myoblasts with myostatin expressing constructs did rescue MyoD-induced myogenic differentiation. Consistent with this, the treatment of Dodamtang rescued the expression of a MyoD in C2C12 myoblasts treated with myostatin. Taken together, these results suggest that induction of MyoD by Dodamtang inhibits myostatin activity and expression via SMAD3 resulting in the rescue of the myoblasts to differentiate into myotubes. Thus we propose that myostatin action by Dodamtang plays a critical role in myogenic differentiation and that the muscular hyperplasia and hypertrophy seen in animals that blockage of functional myostatin is because of deregulated proliferation and differentiation of myoblasts.
Key words: Dodamtang, myoD, myostatin, myoblast
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