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The Journal of Internal Korean Medicine > Volume 28(1); 2007 > Article
The Journal of Internal Korean Medicine 2007;28(1): 80-91.
Lipopolysaccharide로 산화 스트레스를 유도한 Mouse에서 四補丸의 Peroxynitrite 억제 효과
권율, 정지천
동국대학교 한의과대학 내과학교실
Peroxynitrite Scavenging Activity of Sabohwanin Lipopolysaccharide-Induced Oxidatively-Stressed Mice
Youl Kweon, Ji-cheon Jeong
Dept. of Internal Medicine, College of Korean Medicine, Dongguk University
Correspondence  Ji-cheon Jeong ,Tel: 054-770-1265, Fax: 054-770-1500, Email: jjcjh@paran.com
  Published online: March 30, 2007.
ABSTRACT
Objectives:
Peroxynitrite (ONOO-), superoxide anion radical (?O2-) and nitric oxide (NO) are cytotoxic because they can oxidize several cellular components such as proteins, lipids and DNA. They have been implicated in the aging process, and age-related diseases such as Alzheimer's disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate Sabohwan's activity for scavenging ONOO- and its precursors, NO and ?O2-.

Methods :
For this study, the fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used.

Results:
Sabohwanblocked tert-butylhydroperoxide (t-BHP)-induced cell death in a dose-dependent fashion. It scavenged t-BHP-induced ONOO-, NO and ?O2- in YPEN cells. Sabohwan inhibited the generation of ONOO-, NO and ?O2- in the lipopolysaccharide (LPS)-treated mouse kidney postmitochondria both in vitro and in vivo. The lipid peroxide level increased and glutathione level decreased in the LPS-treated mice, whereas the ones in the Sabohwanadministered group among the LPS-treated mice reversed toward their natural levels.

Conclusions:
These results suggest that Sabohwanis an effective ONOO-, ?O2- and NO scavenger, and thereby it might have a potential role as a therapy against the aging process and age-related diseases.
Key words: Sabohwan, lipopolysaccharide, peroxynitrite, nitric oxide, reactive oxygen species
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