Effect of <i>Gagam-Danguieumja</i> through Regulation of MAPK on LPS-Induced
Inflammation in RAW 264.7 Cells

Kim, Tae-yeon

The Journal of Internal Korean Medicine > Volume 34(4); 2013 > Article

Original Article

The Journal of Internal Korean Medicine 2013;34(4): 339-348.

LPS로 유도된 RAW 264.7 cell의 염증반응에서 MAPK 조절에 의한 加減當歸飮子의 항염증 효과

김태연

세명대학교 한의과대학 병리학교실

Effect of Gagam-Danguieumja through Regulation of MAPK on LPS-Induced Inflammation in RAW 264.7 Cells

Tae-yeon Kim

Dept. of Oriental Pathology, College of Korean Medicine, Se-Myung University

Correspondence

Tae-yeon Kim ,Tel: 043-649-1706, Fax: 043-649-1706, Email: violet805@hanmail.net

Published online: December 30, 2013.

ABSTRACT

Objectives:
Danguieumja is a traditional medicinal prescription to treat skin disease. It was commonly used for the treatment of itching, chronic urticaria and atopic dermatitis in Korea by the addition or omission of several herbs. This study investigated the anti-inflammatory potential of Gagam-Danguieumja (GDE) water extract.

Methods :
We examined the effects of GDE on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) in a murine macrophage cell line, RAW 264.7 cells.

Results:
GDE inhibited production of NO in a dose dependent manner and also decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2). As a possible molecular mechanism of anti-inflammatory effect increased phosphorylation of mitogen-activating protein kinases (MAPK) by LPS were blocked by GDE treatment.

Conclusions:
These results suggest that GDE has an anti-inflammatory therapeutic potential through the inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes.

Key words: Gagam-Danguieumja, anti-inflammation, nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, mitogen-activating protein kinase