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The Journal of Internal Korean Medicine 2007;28(1): 166-175. |
| 大薊와 실리비닌의 Mouse BV2 Microglial Cells에서 Lipopolysaccharide에 의해 유발된 염증반응에 대한 신경보호 효과 |
| 여현수, 김동우, 전찬용, 최유경, 박종형 |
| 경원대학교 한의과대학 내과학교실 |
| Neuroprotective Effect of Cirsium japonicum and Silibinin on Lipopolysaccharide-induced Inflammation in BV2 Microglial Cells |
| Hyun-soo Yeo, Dong-woo Kim, Chan-yong Jun, You-kyung Choi, Chong-hyeong Park |
| Department of Internal Medicine, College of Oriental Medicine, Kyungwon University |
| Correspondence |
Chong-hyeong Park ,Tel: 02-425-3456, Fax: 02-425-3560, Email: sdragon@hitel.net
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Published online: March 30, 2007. |
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| ABSTRACT |
Objectives:
This study was designed to evaluate the neuroprotective effect of Cirsium japonicum and Silibinin on lipopolysaccharide-induced inflammation in BV2 microglial cells.
Methods :
We studied on the neuroprotective effect of lipopolysaccharide-induced inflammation using MTS assay, western blot, and nitric oxide detection on mouse BV2 microglial cells.
Results:
Cirsium japonicum dose-dependently (50㎍/ml∼250㎍/ml) inhibited nitrite production and iNOS expression in lipopolysaccharide-induced BV2 microglia and also significantly reduced lipopolysaccharide-induced COX-2 activation in western blot. Silibinin dose-dependently (10μM∼100μM) inhibited nitrite production and iNOS expression in lipopolysaccharide-induced BV2 microglial cells. Silibinin also significantly reduced lipopolysaccharide-induced COX-2 activation in western blot.
Conclusion:
These effects of neuroprotection related to anti-inflammation suggest that Cirsium japonicum and Silibininmay be useful candidates for the development of a drug for related neurodegenerative diseases. |
| Key words:
Cirsium japonicum, Silibinin, BV2 Microglial Cells, Oriental Herb-Medicine |
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