| 澤瀉가 유리지방산으로 유발된 HepG2 cell의 lipoapoptosis에 미치는 영향 |
| 김은영, 이장훈 |
| 경희대학교 한의과대학 간계내과학교실 |
| The Effect of Alisma orientale Extract on Free Fatty Acid-induced
Lipoapoptosis in HepG2 Cells |
| Eun-young Kim, Jang-hoon Lee |
| Dept. of Internal Medicine, College of Korean Medicine, Kyung-Hee University |
| Correspondence |
Jang-hoon Lee ,Tel: 02-958-9118, Fax: 02-958-9258, Email: komclive@khu.ac.kr
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Published online: June 30, 2014. |
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| ABSTRACT |
Objectives:
This study was designed to investigate the effect on lipoapoptosis of Alisma orientale extract against free fatty acid-induced cellular injury.
Methods :
HepG2 cells were used in an vitro model. HepG2 cells were treated with free fatty acids to generate a cellular model of nonalcoholic fatty liver disease (NAFLD). Using this cellular model, the anti-apoptotic effect and reducing steatosis of Alisma orientale extract against free fatty acid-induced cellular injury was evaluated by measuring steatosis and apoptosis.
Results:
Alisma orientale extract significantly attenuated free fatty acid-induced intracellular steatosis. Alisma orientale extract inhibited free fatty acid-mediated activation of pJNK, PUMA, BAX, caspase-3, and –9, and apoptotic kinases that are correlated with NAFLD. Alisma orientale extract also promoted Bcl-2, a anti-apoptotic protein.
Conclusions:
From the above, the Alisma orientale extract decreased the hepatocyte steatosis and showed the hepatocelluar protective effect by the regulation of apoptosis-related protein. It proposes the possibility of Alisma orientale extract to the treatment of nonalcoholic fatty liver disease in clinics. |
| Key words:
nonalcoholic fatty liver disease (NAFLD), Alisma orientale, lipoapoptosis, HepG2 cell, free fatty acids (FFAs) |
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