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The Journal of Internal Korean Medicine > Volume 36(4); 2015 > Article
The Journal of Internal Korean Medicine 2015;36(4): 486-497.
황련-감초 추출물을 이용한 하태독법이 NC/Nga 생쥐에서 유발된 아토피 유사 피부염에 미치는 항염증 효과
차호열1, 안상현2, 정아람1, 천진홍1, 박선영3, 최준용4, 김기봉1
1부산대학교 한방병원 한방소아과
2부산대학교 한의과학연구소
3세명대학교 한의과대학 생리학교실
4부산대학교 한방병원 한방내과
Anti-inflammatory Effects of Hataedock Extracted from Coptidis Rhizoma and Glycyrrhiza Uralensis on Atopic Dermatitis-like Skin Lesions of NC/Nga Mouse
Ho-yeol Cha1, Sang-hyun Ahn2, A-ram Jeong1, Jin-hong Cheon1, Sun-young Park3, Jun-yong Choi4, Ki-bong Kim1
1Dept. of Pediatrics of Korean Medicine, Pusan National University Hospital
2Research Institute for Korean Medicine, Pusan National University
3Dept. of Physiology, College of Korean Medicine, Se-Myung University
4Dept. of Internal Medicine of Korean Medicine, Pusan National University Hospital
Correspondence  Ki-bong Kim ,Tel: +82-55-360-5952, Fax: +82-55-360-5952, Email: kkb2630@gmail.com
Received: November 2, 2015,   Accepted: December 4, 2015,   Published online: December 31, 2015.
ABSTRACT
Objectives:
Hataedock is an orally administered herbal extract treatment for newborn babies that dispels toxic heat and meconium gathered by the fetus. The purpose of this study was to evaluate whether Hataedock alleviates inflammatory skin damage in AD (Atopic Dermatitis)-induced NC/Nga mice through regulating and maintaining the skin barrier and anti-inflammation effects.

Methods:
We established an AD model in three-week-old NC/Nga mice through the repeated application of DNFB (dinitrochlorobenzene) on days 28, 35, and 42 after Hataedock treatment was orally administered. We identified changes in the skin barrier and anti-inflammation effects through the histological and immunohistochemical changes of TNF-α, NF-κB p65, iNOS, COX-2, and apoptotic bodies.

Results:
Skin damage and angiogenesis were mitigated in the HT (Hataedock) group. Damage to the intercellular space of the stratum corneum as well as hyperplasia, edema, the infiltration of lymphocytes, and the increase of capillaries decreased in the HT group. Our results suggest that Hataedock treatment significantly down-regulated levels of TNF-α by 38% (p<0.001) and of NF-κB p65 by 70% (p<0.001). But Hataedock up-regulated apoptosis by 183% in dermatitis-induced skin.

Conclusions:
These results suggest that Hataedock alleviates AD through diminishing the various inflammatory cytokines in skin lesions that are involved in the initial steps of AD development. It might have potential applications for the prevention and treatment of atopic dermatitis.
Key words: Hataedock, atopic dermatitis, TNF-α, NF-κB p65, apoptosis
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