Effect of <i>Yanggyuksanhwa-tang</i> on c-Fos and c-Jun Expression in Ischemic Damaged Hippocampus of Aged BCAO Rats

Kim, Seung-Jun; Shin, Jung-Won; Sohn, Youngjoo; Jung, Hyuk-Sang; Won, Ran; Sohn, Nak-Won

The Journal of Internal Korean Medicine > Volume 24(2); 2003 > Article

Original Article

The Journal of Internal Korean Medicine 2003;24(2): 337-347.

노령 흰쥐의 뇌허혈 손상시 凉膈散火湯이 뇌해마의 c-Fos 및 c-Jun 발현에 미치는 영향

Effect of Yanggyuksanhwa-tang on c-Fos and c-Jun Expression in Ischemic Damaged Hippocampus of Aged BCAO Rats

Seung-Jun Kim, Jung-Won Shin, Youngjoo Sohn, Hyuk-Sang Jung, Ran Won, Nak-Won Sohn

¹Department of Anatomy, College of Oriental Medicine, Kyung Hee University
²Department of Neuroscience. Graduate School of East-West Medical Science, Kyung Hee University

Correspondence

Nak-Won Sohn ,Tel: 031-201-2747, Fax: 031-204-8119, Email: sohnnw@khu.ac.kr

Received: April 17, 2003, Accepted: June 16, 2003, Published online: June 30, 2003.

ABSTRACT

This study investigated the effect of Yanggyuksanhwa-tang on cerebral ischemia of the rats. Considering age-related impact on cerebral ischemia, aged rats (18 months old) were used for this study. Ischemic damage was induced by the transient occlusion of bilateral common carotid arteries (BCAO) under the hypotension. Yanggyuksanhwa-tang was administered twice orally. Then changes of immunohistochemical expression of c-fos and c-jun in ischemic damaged hippocampus were observed. The BCAO in aged rats led significant increase of c-fos expression in CA1 and DG of hippocampus. While the treatment of Yanggyuksanhwa-tang significantly attenuated the increase of c-fos expression in CA1 hippocampus following BCAO ischemia. Depending on changes of the normalized optical density (NOD) of immunohistochemical c-fos expression, the treatment of Yanggyuksanhwa-tang significantly attenuated the increase of NOD in CA1 and DG of hippocampus. And there was not changes in CA2 and CA3 hippocampus with respect to the control BCAO group. The BCAO in aged rats led significant increase of c-jun expression in CA1 hippocampus. While the treatment of Yanggyuksanhwa-tang significantly attenuated the increase of c-jun expression in CA1 hippocampus following BCAO ischemia. Depending on changes of the NOD of immunohistochemical c-jun expression, the treatment of Yanggyuksanhwa-tang significantly attenuated the increase of NOD in CA1 hippocampus. And there was not changes in CA2, CA3 and DG of hippocampus with respect to the control BCAO group.

Key words: Aging, Ischemia, c-Fos, c-Jun, Yanggyuksanhwa-tang, rat