| 사군자탕이 H2O2 에 의한 배양심근세포고사에 미치는 영향 |
| 허윤1, 박상범2, 차용석1, 윤종민1, 임준모1, 이주헌2, 나헌식2, 송영준2, 문병순1 |
1원광대학교 한의학전문대학원
2원광대학교 한의과대학 심계내과학교실 |
| Effects of Sagunja-tang Extract on H2O2-induced Cell Apoptosis in Cultured Cardiomyoblast Cells |
| Yun Heo1, Sang-Bum Park2, Yong-seok Cha1, Jong-Min Yun1, Jun-Mo Im1, Joo-Heon Lee2, Heon-Sik Na2, Young-Jun Song2, Byung-Soon Moon1 |
1Professional Graduate School of Oriental Medicine, Wonkwang University
2Department of Circulatory Internal Medicine, College of Oriental Medicine, Wonkwang Univercity |
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Received: September 3, 2002, Accepted: October 4, 2002, Published online: December 30, 2002. |
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| ABSTRACT |
Objective :
The water extract of Sagunja-tang(SGJT) has been traditionally used for treatment of ischemic heart and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of SGJT rescues cells from these damages.
Methods :
This study was designed to investigate the protective mechanism of SGJT on H2O2-induced toxicity in H9c2 cardiomyoblast cells.
Results:
Treatment of H2O2 markedly induced death of H9c2 cardiomyoblast cells with a dosage and time dependent manner. The characteristics of H2O2-induced death of H9c2 showed apparent apoptotic features such as chromatin fragmentation and DNA fragmentation. However, SGJT significantly reduced both H2O2-induced cell death and chromatin fragmentation. The decrease of Bcl2 expression by H2O2 were inhibited by SGJT. In addition, the increase of Bax expression was also inhibited by SGJT. In addition, SGJT have protective effects on H2O2-induced cytotoxicity, which was revealed as apoptosis characterized by the loss of mitochondrial membrane potential in H9c2 cardiomyoblast cells.
Conclusions:
Taken together, this study suggested that SGJT might play a protective role in oxidative damage induced by H2O2. |
| Key words:
Sagunja-tang(SGJT), H2O2, H9c2 cardiomyoblast cells |
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