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The Journal of Internal Korean Medicine > Volume 28(2); 2007 > Article
Original Article
The Journal of Internal Korean Medicine 2007;28(2): 230-241.
Protective Effects of Phenolic-rich Fraction(PRF) from Fructus Schisandrae on H2O2-induced Apoptosis of SH-SY5Y Cells
In-hwan Son, Key-sang Lee
Dept. of International Medicine of Oriental Medicine Hospital, Wonkwang Univ.
Correspondence  Key-sang Lee ,Tel: 031-390-2670, Email: keysang@wonkwang.ac.kr
  Published online: June 30, 2007.
ABSTRACT
Objective :
This study was intended to ascertain the protective effect of phenolic-rich fraction (PRF) from Fructus Schisandrae on SH-SY5Y cells.

Methods :
PRF was obtained from the 80% ethanol extract of Fructus Schisandrae by Sepabeads SP-850 column chromatography. The neuroprotective effect of the FS PRF was investigated due to the hydrogen peroxide (H2O2)-induced apoptosis of cultured SH-SY5Y cells.

Results:
Cell viability assays revealed that pretreating SH-SY5Y cells with PRF (10-200 μg/mL) resulted in significant dose-dependent protection against H2O2-induced cell death. The effect was assessed by flow cytometric analysis of DNA contents using propidium iodide (PI) staining. The population of apoptotic cells was increased by 32.89% in only H2O2 (150 μM)-treated environment, but it was reduced by pre-treatment of FS PRF (200 μg/mL) to 21.61%. H2O2-induced caspase-3 activation and PARP cleavage were reduced in FS PRF pre-treated cells, and PRF led to an apparent suppressive effect on the oxidative stress induced by reactive oxygen species (ROS).

Conclusion:
This study showed that Fructus Schisandrae should be useful for the treatment and prevention of neurodegenerative diseases associated with elevated ROS levels.
Key words: Fructus Schisandrae, PRF, SH-SY5Y, H2O2, Apoptosis
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