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The Journal of Internal Korean Medicine > Volume 28(3); 2007 > Article
Original Article
The Journal of Internal Korean Medicine 2007;28(3): 409-420.
석웅황의 시험관내 위암, 신경교종 및 전립선암 세포에 대한 항암 연구
김선량, 류봉하, 윤성우
경희대학교 한의과대학 비계내과학 교실
Anti-tumor effects of Realgar on Stomach Cancer Cells (AGS), Glioma Cells (T98G, A172, SNU-489) and Prostate Cancer Cells (LNCaP)
Seon-ryang Kim, Bong-ha Ryu, Seong-woo Yoon
3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University
Correspondence  Bong-ha Ryu ,Tel: 02-958-9137, Fax: 02-958-9136, Email: rbongh@khu.ac.kr
  Published online: September 30, 2007.
ABSTRACT
Objectives:
The purpose of this study was to identify the anti-tumor effects of realgar on various cancer cells through molecular biologic and cellular biologic methods.

Materials & Methods :
We used 5 kinds of cancer cell lines:stomach cancer cells (AGS), glioma cells (T98G, A172, SNU-489) and prostate cancer cells (LNCaP). We injected the boiled extract of realgar, 50㎍/ml and 100㎍/ml to culture media (㎖) for 24 hours. We examined the morphological changes under an inverted microscope and a fluorescence microscope. We measured the suppressive effect on viability of 5 kinds of cancer cells via XTT assay. We examined the effect on the revelation of PARP cleavage, Bcl-2 protein and Bax protein by western blot analysis.

Results:
The extract of realgar caused markedly morphological changes on AGS, T98G, SNU-489, and LNCaP. All of them showed withdrawn and floating appearance. The suppressive effect on viability of AGS, T98G, A172, SNU-489, and LNCaP showed that each test group had more suppressive effect on viability of AGS, T98G, A172, SNU-489, and LNCaP than the control group, which was statistically significantly (p<0.01). The extract of realgar did not induce PARP cleavage in AGS, T98G, A172, SNU-489, or LNCaP. In the revelation of protein related to apoptosis, the protein levels of Bcl-2 decreased and the protein levels of Bax increased in AGS, T98G, SNU-489, and LNCaP treated with realgar. The protein levels of Bcl-2 decreased and the protein levels of Bax did not change in A172 treated with realgar.

Conclusions:
This experiment showed that realgar has anti-tumor effect on stomach cancer cells (AGS), glioma cells (T98G, SNU-489), and prostate cancer cells (LNCaP)
Key words: realgar, cancer, stomach cancer cells, glioma cells, prostate cancer cells
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