Induction of Apoptosis by Curcuma aromatica on Lung Cancer Cells(A549), Cervical Cancer Cells(HeLa), Glioma Cancer Cells(A172) and Prostate Cancer Cells(PC3)

Park, Sanghyun; Ryu, Bongha; Kim, Jinsung; Yoon, Sanghyub

The Journal of Internal Korean Medicine > Volume 27(2); 2006 > Article

Original Article

The Journal of Internal Korean Medicine 2006;27(2): 379-393.

鬱金이 肺癌, 子宮癌, 神經膠腫 및 前立腺癌에 대한 細胞自殺誘導에 미치는 影響

박상현, 류봉하, 김진성, 윤상협

경희대학교 한의과대학 비계내과학교실

Induction of Apoptosis by Curcuma aromatica on Lung Cancer Cells(A549), Cervical Cancer Cells(HeLa), Glioma Cancer Cells(A172) and Prostate Cancer Cells(PC3)

Sanghyun Park, Bongha Ryu, Jinsung Kim, Sanghyub Yoon

3rd Deptartment of Internal Medicine, College of Oriental Medicine, Kyunghee University

Correspondence

Bongha Ryu ,Tel: 02-958-9140, Fax: 02-958-9136, Email: rbongh@khu.ac.kr

Received: May 11, 2006, Accepted: June 15, 2006, Published online: June 30, 2006.

ABSTRACT

Objectives:
We are aimed to identify anti-tumor effects of Curcuma aromatica on some kinds of cancer cells through molecular biologic methods. Materials &

Methods:
We used 4 kinds of cancer cell lines such as lung cancer cells(A549), cervical cancer cells(HeLa), glioma cancer cells(A172) and prostate cancer cells(PC3). We treated the boiled extract of Curcuma aromatica 5㎍, 10㎍ to cultural media(㎖) for 24 hours. We measured the cytotoxicity on 4 kinds of cancer cells through tryphan blue exclusion test and the suppressive effect on viability of 4 kinds of cancer cells via MTT assay. We measured change of mitochondria membrane potential via flow cytometry. The quantitative RT-PCR was used to examine the effect on the revelation of Bcl-2 and Bax which are genes related to apoptosis. We examined the effect on the revelation of Bcl-2 protein and Bax protein by western blot analysis.

Results:
In the experiment of tryphan blue exclusion test, the extract of Curcuma aromatica showed more significant killing effect on A549, HeLa than the control group with density dependent manner, which was statistically significant. In the experiment of MTT assay the extract of Curcuma aromatica showed more suppressive effect on viability of A549, HeLa than the control group with density dependent manner, which was statistically significant. Curcuma aromatica induced apoptosis by decreasing the membrane potential of mitochondria in A549, HeLa. In the experiment of the revelation of genes related to apoptosis, the revelation of Bcl-2 decreased and the revelation of Bax increased in A549, HeLa treated with Curcuma aromatica with dose dependent manner. In the experiment of the revelation of protein related to apoptosis, the protein levels of Bcl-2 decreased and the protein levels of Bax increased in A549, HeLa treated with Curcuma aromatica with dose dependent manner.

Conclusions:
From this study, we can infer that Curcuma aromatica has anti-tumor effect on lung cancer cells and uterine carcinoma cells but not on glioma cells and prostate cancer cells.

Key words: Curcuma aromatica, lung cancer cell, cervical cancer cell, glioma cancer cell, prostate cancer cell, apoptosis, Bcl-2, Bax