|
|
The Journal of Internal Korean Medicine 2006;27(4): 888-893. |
| 구조활성상관(QSAR)에 의한 피마엽(蓖麻葉) 추출물의 HIV-1 효소억제활성인자 예측 |
| 한창호1, 유영법2 |
1동국대학교 한의과대학 일산한방병원 한방내과
2한국한의학연구원 한약제제연구부 |
| Inhibitory Effects of Ricinus communis on HIV-1 Essential Enzymes in vitro and Prediction of Inhibitory Factor Using QSAR in silico |
| Chang-ho Han1, Young-beob Yu2 |
1Department of Oriental Internal Medicine, DongGuk University International Hospital, Siksa-dong 814, Ilsandonggu, Goyangsi, Gyeonggido 410-773, Rep. of Korea
2Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Jeonmin-dong 461-24, Yuseong-gu, Daejeon 305-810, Rep. of Korea |
| Correspondence |
Young-beob Yu ,Tel: 042-868-9463, Fax: 042-868-9471, Email: ybyu@kiom.re.kr
|
|
|
Published online: December 30, 2006. |
|
|
|
| ABSTRACT |
Objectives:
For the purpose of developing new anti-HIV agents from natural sources, the extracts of Ricinus communis were tested for their inhibitory effects on essential enzymes reverse transcriptase (RT), protease and alpha-glucosidase. Inhibition activity of major compounds of Ricinus communis were predicted from quantitative structure activity relationships (QSAR) in silico.
Methods and Results :
In the anti-HIV-1 RT using enzyme-linked oligonucleotide sorbent assay (ELOSA) method, water and methanol extracts (100ug/ml) of Ricinus communis showed strong activity of 94.2% and 82.7%, respectively. In the HIV-1 protease and alpha-glucosidase inhibition assay, neither water nor methanol extracts of Ricinus communis inhibited the activity of the enzyme to cleave any substrates as oligopeptides and oligosaccharides.
Conclusions:
We found that for these samples it is possible that the inhibition of the RT in vitro is due to the secondary metabolites of Ricinus communis such as ricinine and quercetin. It would beof great interest to identify the compounds which are responsible for this inhibition, since all therapeutically useful agents up to date are RT inhibitors. |
| Key words:
Ricinus communis, HIV-1 essential enzymes, QSAR |
|
|
|
PDF Links
Full text via DOI 
Download Citation 
