楡根皮 추출물의 활성종 억제 및 염증 촉진 인자 제어 효과 |
조은영, 정지천 |
동국대학교 한방병원 내과 |
The Inhibitory Effects of Ulmus davidiana on the Reactive Species and Proinflammatory Proteins |
Eun-young Jo, Ji-cheon Jeong |
Department of Oriental Internal Medicine, Hospital of Oriental Medicine, Dongguk University |
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Published online: June 30, 2008. |
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ABSTRACT |
Objectives: This study was to investigate the inhibitory effects of Ulmus davidiana on the generation of peroxynitrite
(ONOO-), nitric oxide (NO) and superoxide anion radicals (․O2
-) in the endothelial cells of rat vessels. The effects of Ulmus
davidiana on the expression of inflammation-related proteins, NF-κB (p50, p65), COX-2, and iNOS, were examined by western
blotting.
Methods : For this study, fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2)
and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed via using anti-NF-κB (p50,
p65), anti-COX-2, and anti-iNOS, respectively.
Results: Ulmus davidiana inhibited the generation of ONOO-, NO and ․O2
- in the lipopolysaccharide (LPS)-treated
endothelial cells of rat vessels in vitro. Ulmus davidiana inhibited the expression of COX-2 and iNOS genes by means of
decreasing the NF-κB activation.
Conclusions: These results suggest Ulmus davidiana is effective on inhibiting the generation of ONOO-, NO and ․O2
-,
and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related
diseases. |
Key words:
Ulmus davidiana, peroxynitrite, nitric oxide, superoxide anion radical, NF-κB. |
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