Effects of Salviae Miltiorrhizae Radix, Rhei Rhizoma and Carthami Flos combined with Samgijiwhang-Tang on Streptozotocin-induced Diabetic Nephropathic Rats

Cho, Hyun-joo; Kim, Yong-seung

The Journal of Internal Korean Medicine > Volume 26(4); 2005 > Article

Original Article

The Journal of Internal Korean Medicine 2005;26(4): 767-775.

蔘芪地黃湯加丹蔘,大黃,紅花가 Streptozotocin으로 유발된 생쥐의 糖尿病性腎症에 미치는 영향

조현주, 김용성

동신대학교 한의과대학 신계내과학 교실

Effects of Salviae Miltiorrhizae Radix, Rhei Rhizoma and Carthami Flos combined with Samgijiwhang-Tang on Streptozotocin-induced Diabetic Nephropathic Rats

Hyun-joo Cho, Yong-seung Kim

Department of Internal Medicine, College of Oriental Medicine, Dongshin University

Correspondence

Hyun-joo Cho ,Tel: 043-743-1675, Email: moon5303@nate.com

Received: August 3, 2005, Accepted: September 8, 2005, Published online: December 31, 2005.

ABSTRACT

This study was carried out to investigate the preventive effect of Salviae Miltiorrhizae Radix, Rhei Rhizoma and Carthami Flos combined with Samgijiwhang-Tang(SJTSRC) on streptozotocin(STZ)-induced diabetic nephropathy. Rats were divided into a control group of rats with STZ-induced diabetic nephropathy, a sample group of those given SJTSRC, and a normal group. In this experiment diabetic nephropathy was induced by giving STZ(60㎎/㎏) to rats via the peritoneum, and effects were assessed with measures of serum creatinine, serum BUN, secretion content of albumin and glucose content of urine, malondialdehyde(MDA) and glutathione(GSH) content in cortex of kidney. When STZ was injected into sample rat, the value of creatinine and BUN increased validly and STZ did damage to the kidney. When applying SJTSRC to sample rats, the value of serum creatinine decreased validly but the value of serum BUN decreased invalidity. It was confirmed that SJTSRC had an effect on recovery after kidney damage and secretion content of albumin increasedafter administration of SJTSRC but there was no change in glucose content of urine compared with the control group. The decrease of secretion of albumin after injection of STZ was taken to mean progressive diabetic nephropathy, and that reversal of that trend after SJTSRC administration showed that kidney function had improved, not through decreasing blood sugar, but through other factors. Results suggest that diabetic nephropathy was induced by STZ, and SJTSRC was effective in restricting the extent of damage to the kidney and halting the progression of diabetic nephropathy with improvement in levels of serum creatinine and albumin secretion. More study is needed, particularily pertaining to anti-oxidative effects in the kidney cortex.