Effects on Alzheimer’s disease by <i>Jimitang</i> in CT105-overexpressed SK-N-SH cell lines

Kang, Sung-Jun; Park, Chi-Sang; Park, Chang-Gook

The Journal of Internal Korean Medicine > Volume 25(3); 2004 > Article

Original Article

The Journal of Internal Korean Medicine 2004;25(3): 482-491.

CT105로 유도된 신경모세포종 세포주에서 指迷湯의 항치매효과

강승준, 박치상, 박창국

대구한의대 한의과대학 심계내과학교실

Effects on Alzheimer’s disease by Jimitang in CT105-overexpressed SK-N-SH cell lines

Sung-Jun Kang, Chi-Sang Park, Chang-Gook Park

Department of Circulatory Internal Medicine, College of Oriental Medicine, Daeguhaany University

Correspondence

Chi-Sang Park ,Tel: 053-770-2104, Email: stephano@dhu.ac.kr

Received: July 19, 2004, Accepted: August 5, 2004, Published online: September 30, 2004.

ABSTRACT

Alzheimer’s disease(AD) is a geriatric dementia that is widespread in old age. In the near future AD may be the biggest problem in public health service. Although a variety of oriental therapies in the study of Jimitang have been traditionally utilized for the treatment of AD, their pharmacological effects and active mechanisms have not been fully elucidated. This study is an investigation of effects of Jimitang on apoptotic cell death induced by CT105 overexpression in SK-N-SH neuroblastoma cell lines DNA fragmentation, neurite outgrowth assay and LDH activity assay were examined. The regeneratory and inhibitory effects on Alzheimer’s disease in pCT105-induced neuroblastoma cell lines by Jimitang water extract were examined. Findings from these experiments have shown that Jimitang inhibits the synthesis or activities of CT105, which has neurotoxicities and apoptotic activities in cell lines. In addition, pretreatment of Jimitang(>50 ㎍/mL for 12 hours) partially prevented CT(105)-induced cytotoxicity in SK-N-SH cell lines, and were inhibited by pretreatment. Jimitang(>50 ㎍/mL for 12 hours) repaired CT(105)-induced neurite outgrowth when SKN- SH cell lines were transfected with CT(105). Results of this study show that, in the Jimitang group, the apoptosis in the nervous system is inhibited, the repair against the degerneration of neuroblastoma cells by CT105 expression is promoted. In addition, Jimitang was found to inhibit DNA fragmentation induced by CT105 overexpression, and promote neurite outgrowth. These findings suggest that Jimitang is beneficial for the treatment of AD.

Key words: Jimitang, CT105, SK-N-SH cell lines, Alzheimer’s disease.