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The Journal of Internal Korean Medicine 2003;24(1): 113-122. |
| Lipopolysaccharide 로 유발된 HepG2 세포의 염증반응에 대한 가감청간탕의 효과에 대한 연구 |
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| The effect of Gagamchunggan-tang on lipopolysaccharide-induced expression of NF κ-B downstream genes in HepG2 cell |
| Sung-Hwan Kim, Sang-Ho Seo, Sang-Hoon Hong |
| Department of Internal Medicine, Coollege of Oriental Medicine Dongeui University |
| Correspondence |
Sang-Hoon Hong ,Tel: 051-850-8810, Fax: 051-867-5162, Email: shhong@demc.or.kr
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Received: January 8, 2003, Accepted: February 7, 2003, Published online: March 30, 2003. |
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| ABSTRACT |
Objective :
The aim of this study was to evaluate the efficacy of Gagamchunggan-tang on anti-inflammation reaction with lipopolysaccharide (LPS)-induced HepG2 cell.
Method:
We examined the effects of the Gagamchunggan-tang , a traditional drug for liver inflammation, on the process of lipopolysaccharide (LPS)-induced nuclear factor- κBp65 (NF-κBp65) activation in HepG2 cell. SDS-PAGE, Western blotting, Immunofluorescence staining were studied.
Results:
Immunoblot analysis showed that the level of nucleic NF-κBp65 was rapidly up-regulated and cytosolic inhibitory I-κBα was down-regulated by LPS challenge. While Gagamchunggan-tang inhibited an increase of NF-κBp65 and degradation of I- κBα in HepG2 cell. Besides LPS-induced expression of a group of genes, such as tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), are repressed by Gagamchunggan-tang. It may be concluded that Gagamchunggan-tang attenuates the progress of LPS-induced inflammation by reduction of NF-κBp65 activation.
Conclusion:
The Gagamchunggan-tang would be useful as a therapeutic agent for endotoxin-induced liver disease. |
| Key words:
Gagamchunggan-tang, LPS, NF- Bp65 |
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