|
|
The Journal of Internal Korean Medicine 2003;24(1): 104-112. |
| 蘇合香元이 저산소증 유발 배양 대뇌신경세포에 미치는 영향 |
|
|
| The Effect of Sohaphyang-won’s for Delayed Neuronal Death in Hypoxia |
| Ji-Hun Lee1, Kyoung-Sun Yun1, Sung-Hyun Jeong1, Gil-Cho Shin1, Won-Chu Lee1, Il-Su Moon2 |
1Department of Internal Medicine, College of Oriental Medicine, Dongguk University
2Department of Anatomy, College of Medicine, Dongguk University |
|
|
|
Received: January 2, 2003, Accepted: February 11, 2003, Published online: March 30, 2003. |
|
|
|
| ABSTRACT |
Objectives:
The purpose of this study is to evaluate the effects of Sohaphyang-won and is to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20(E20) cortical cells of a guinea pig(Sprague Dawley).
Methods :
E20 cortical cells, used in this investigation were dissociated in Neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Sohaphyang-won was added to the culture media for 72 hours. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Sohaphyang-won’s effects for neuronal death protection were evaluated by LDH assay and the mechanism was studied by Bcl-2, Bak, Bax, caspase family.
Results:
This study indicates that Sohaphyang-won’s effects for neuronal death protection in hypoxia is confirmed by LDH assay by the method of Embryonic day 20(E20) cortical neuroblast.
Conclusions:
Sohaphyang-won’s mechanism for neuronal death protection in hypoxia restrains inflow of cytochrome C into cellularity caused by Bcl-2 increase and reduces the caspase cascade initiator caspase-10 and the effector caspase-3. |
| Key words:
Sohaphyang-won, hypoxia, Bcl-2, caspase |
|
|
|
PDF Links
Full text via DOI 
Download Citation 
