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The Journal of Internal Korean Medicine 2002;23(3): 396-405. |
| 황금이 저산소증으로 유발된 대뇌신경세포의 세포사에 미치는 영향 |
| 김경훈1, 정수현1, 김재우1, 엄형섭1, 정승현1, 신길조1, 이원철1, 문일수2 |
1동국대학교 한의과대학 내과학 교실
2동국대학교 의과대학 해부학교실 |
| Department of Internal Medicine, College of Oriental Medicine, Dongguk University |
| Kyong-Hoon Kim1, Su-Hyeon Jeong1, Jae-Woo Kim1, Hyung-Sup Eum1, Sung-Hyun Chung1, Gil-Cho Shin1, Won-Chul Lee1, Il-Soo Moon2 |
1Department of Internal Medicine, College of Oriental Medicine, Dongguk University
2Department of Anatomy, College of Medicine, Dongguk University |
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Received: July 29, 2002, Accepted: August 31, 2002, Published online: September 30, 2002. |
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| ABSTRACT |
Objectives:
This study was carried out to investigate the effect of HwangGum(Scutellariae Radix) extracts on cultured brain neuronal cells in hypoxia.
Methods :
Embryonic day 20 (E20) cortical cells of a rat (Sprague Dawley). The E20 cortical cells were dissociated in Neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, HwangGum was added to the culture media for 72 hrs. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, HwangGum s effects for neuronal death protection were evaluated by LDH assay and the mechanisms of protection were studied by Bcl-2, Bak, Bax, caspase family.
Results:
HwangGum s mechanisms for neuronal death protection in hypoxia are to increases the anti-apoptosis protein Bcl-2 along with other proapoptotic proteins. Interestingly, caspase-3, the effector, is also increased significantly. Despite the increase in caspase-3, the HwangGum exhibited neuroprotective effects in hypoxia.
Conclusions:
HwangGum protects delayed neuronal death in hypoxia by increasing Bcl-2 and reducing caspase-3 activity by mechanisms as yet unknown. |
| Key words:
HwangGum, hypoxia, Bcl-2, Bax, Bak |
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