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The Journal of Internal Korean Medicine > Volume 22(1); 2001 > Article
The Journal of Internal Korean Medicine 2001;22(1): 87-93.
인진호가 Hep G2 세포에서 에탄올 매개성 Cytokine분비에 미치는 영향
최수덕, 심정섭, 김일환, 김강산, 강병기
원광대학교 한의과대학 간계내과학교실
The Effect of Artemisia Capillaris Herba on Ethanol-Induced Cytokines (TNF-α , IL-1α ) Secretion in Hep G2 Cells
Su-Deock Choi, Jung-Sub Sim, Il-Hwan Kim, Gang-San Kim, Byung-Ki Kang
Department of Internal Medicine, College of Oriental Medicine, Wonkwang University
Correspondence  Su-Deock Choi ,
Received: May 12, 2001,   Accepted: June 12, 2001,   Published online: March 30, 2001.
ABSTRACT
A human hepatoma cell line, Hep G2 cells, is reliable for the study of alcohol-induced hepatotoxicity. The aim of this study is to determine the relationship between TNF-α , IL-1α production and EtOH-induced cytotoxicity on Hep G2 cells. The cells were incubated with EtOH in the presence of Artemisia Capillaris Herba(AC) for 24 hours and in the absence of AC for 48 hours. Cytoviability and cytokines release were analyzed by MTT assay and enzyme linked immunosorbent assay (ELISA), respectively. After 24 hours of EtOH exposure, the cytoviability had markedly decreased, and the release of cytokines had increased. The increased amount of cytokines contributed to EtOH-induced cytotoxicity. Anti-TNF-α and IL-1α antibodies almost abolished it. Interestingly, EtOH-induced cytotoxicity and cytokines production were inhibited by AC. Moreover, when AC was used in combination with antibodies, there was a marked inhibition of EtOH-induced cytotoxicity. These results suggest that EtOH-induced cytotoxicity may regulate, by various factors, and AC may prevent the cytotoxicity through partial inhibition of the TNF-α and IL-1α secretion.
Key words: "인진호, Hep G2 cell, 간독성, TNF-α, IL-1α
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