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The Journal of Internal Korean Medicine 2015;36(3): 217-227. |
| LC-MS/MS 및 ICP를 이용한 당뇨환의 화학적 특성 분석 |
| 인정도1, 임대식2, 문승호3, 김원일1 |
1동의대학교 한의과대학 내과학교실
2순천향대학교 자연과학대학 화학과
3(주)아이씨비 |
| Characteristic Effects of Dangnyo-hwan for Diabetes Control Studied Using
LC-MS/MS and ICP |
| Jeong-do In1, Dai-sig Im2, Seung-ho Moon3, Won-ill Kim1 |
1Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dong-Eui University
2Dept. of Chemistry, College of Natural Sciences, Soonchunhyang University
3R&D Center, ICB |
| Correspondence |
Won-ill Kim ,Tel: 051-850-8630, Fax: 051-867-5162, Email: omdstar@deu.ac.kr
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Received: June 19, 2015, Accepted: September 26, 2015, Published online: September 30, 2015. |
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| ABSTRACT |
Objectives:
The primary aim of this study was to identify bioactive compounds in Dangnyo-hwan, a Korean herbal medicine, through instrumental analysis using LC-MS/MS and ICP, and investigate its potential use in diabetes treatment.
Methods:
The extract of Dangnyo-hwan has 12 medicinal herbs; these were compared with 18 marker substances selected from literature survey.
Results:
LC-MS/MS analysis could detect 9 of the 18 bioactive compounds: citruline, catalpol, berberine, ginsenoside Rb1, ginsenoside Rg1, oleanolic acid, β-sitosterol, mangiferin, and schizandrin. While harmful heavy metals such as As, Pb, Cd, Hg, Ni, and Cu were not present in high concentrations, Zn concentration was 4.2 mg in 100 g Dangnyo-hwan.
Conclusions:
Instrumental analysis such as LC-MS/MS and ICP was successfully used to identify bioactive compounds in Dangnyo-hwan. Detection of 9 bioactive substances and Zn from the herb medicine is a valuable finding, and suggests that Dangnyo-hwan is a candidate medicine for diabetes. Further investigations like in vitro assay, percent GPR 119 activity, and percent human DGAT-1 inhibition are underway. |
| Key words:
Dangnyo-hwan, diabetes, LC-MS/MS, ICP, marker substances |
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