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The Journal of Internal Korean Medicine > Volume 36(3); 2015 > Article
The Journal of Internal Korean Medicine 2015;36(3): 217-227.
LC-MS/MS 및 ICP를 이용한 당뇨환의 화학적 특성 분석
인정도1, 임대식2, 문승호3, 김원일1
1동의대학교 한의과대학 내과학교실
2순천향대학교 자연과학대학 화학과
Characteristic Effects of Dangnyo-hwan for Diabetes Control Studied Using LC-MS/MS and ICP
Jeong-do In1, Dai-sig Im2, Seung-ho Moon3, Won-ill Kim1
1Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dong-Eui University
2Dept. of Chemistry, College of Natural Sciences, Soonchunhyang University
3R&D Center, ICB
Correspondence  Won-ill Kim ,Tel: 051-850-8630, Fax: 051-867-5162, Email: omdstar@deu.ac.kr
Received: June 19, 2015,   Accepted: September 26, 2015,   Published online: September 30, 2015.
The primary aim of this study was to identify bioactive compounds in Dangnyo-hwan, a Korean herbal medicine, through instrumental analysis using LC-MS/MS and ICP, and investigate its potential use in diabetes treatment.

The extract of Dangnyo-hwan has 12 medicinal herbs; these were compared with 18 marker substances selected from literature survey.

LC-MS/MS analysis could detect 9 of the 18 bioactive compounds: citruline, catalpol, berberine, ginsenoside Rb1, ginsenoside Rg1, oleanolic acid, β-sitosterol, mangiferin, and schizandrin. While harmful heavy metals such as As, Pb, Cd, Hg, Ni, and Cu were not present in high concentrations, Zn concentration was 4.2 mg in 100 g Dangnyo-hwan.

Instrumental analysis such as LC-MS/MS and ICP was successfully used to identify bioactive compounds in Dangnyo-hwan. Detection of 9 bioactive substances and Zn from the herb medicine is a valuable finding, and suggests that Dangnyo-hwan is a candidate medicine for diabetes. Further investigations like in vitro assay, percent GPR 119 activity, and percent human DGAT-1 inhibition are underway.
Key words: Dangnyo-hwan, diabetes, LC-MS/MS, ICP, marker substances
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