| 사람 신경모세포종 SH-SY5Y 세포주의 파킨슨 모델에 대한 小續命湯의 보호효과 |
| 우찬, 유주연, 장철용, 김효린, 신용진, 문아지, 신선호 |
| 원광대학교 한의과대학 내과학교실 |
| Protective Effects of Sosokmyoung-tang Against Parkinson’s Model
in Human Neuroblastoma SH-SY5Y Cells |
| Chan Woo, Ju-yeon You, Chul-yong Jang, Hyo-rin Kim, Yong-jeen Shin, A-ji Moon, Sun-ho Shin |
| Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University |
|
|
|
Published online: September 30, 2014. |
|
|
|
| ABSTRACT |
Objectives:
In this study we made an effort to investigate the protective effect of SSMT on the N-methyl-4-phenyl-1,2,3,6 -tetrahydropyridine (MPTP) -induced cytotoxicity of SH-SY5Y cells.
Methods:
The cell viability was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MMT) assay. The fluorescence intensity was measured by using a dye and then with propidium iodide (PI) DNA flow cytometry analysis of the effects on the cell cycle of the SH-SY5Y cells and were used to measure the fluorescence of intracellular reactive oxygen species generation by MPTP.
Results:
Pretreatment of SSMT significantly suppressed MPTP-induced cytotoxicity, which was revealed as apoptosis characterized by the reduction of cell viability, the increase of ROS production, and the loss of mitochondrial membrane potential in SH-SY5Y cells.
Conclusions:
These findings suggest that SSMT exerts neuroprotective effects on human neuroblastoma SH-SY5Y cells by MPTP-induced dopaminergic neurodegeneration. |
| Key words:
SH-SY5Y cell, neuroprotective effect, Sosokmyoung-tang (SSMT) |
|
PDF Links
Full text via DOI 
Download Citation 
