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The Journal of Internal Korean Medicine > Volume 35(2); 2014 > Article
The Journal of Internal Korean Medicine 2014;35(2): 184-194.
澤瀉가 유리지방산으로 유발된 HepG2 cell의 lipoapoptosis에 미치는 영향
김은영, 이장훈
경희대학교 한의과대학 간계내과학교실
The Effect of Alisma orientale Extract on Free Fatty Acid-induced Lipoapoptosis in HepG2 Cells
Eun-young Kim, Jang-hoon Lee
Dept. of Internal Medicine, College of Korean Medicine, Kyung-Hee University
Correspondence  Jang-hoon Lee ,Tel: 02-958-9118, Fax: 02-958-9258, Email: komclive@khu.ac.kr
  Published online: June 30, 2014.
ABSTRACT
Objectives:
This study was designed to investigate the effect on lipoapoptosis of Alisma orientale extract against free fatty acid-induced cellular injury.

Methods :
HepG2 cells were used in an vitro model. HepG2 cells were treated with free fatty acids to generate a cellular model of nonalcoholic fatty liver disease (NAFLD). Using this cellular model, the anti-apoptotic effect and reducing steatosis of Alisma orientale extract against free fatty acid-induced cellular injury was evaluated by measuring steatosis and apoptosis.

Results:
Alisma orientale extract significantly attenuated free fatty acid-induced intracellular steatosis. Alisma orientale extract inhibited free fatty acid-mediated activation of pJNK, PUMA, BAX, caspase-3, and –9, and apoptotic kinases that are correlated with NAFLD. Alisma orientale extract also promoted Bcl-2, a anti-apoptotic protein.

Conclusions:
From the above, the Alisma orientale extract decreased the hepatocyte steatosis and showed the hepatocelluar protective effect by the regulation of apoptosis-related protein. It proposes the possibility of Alisma orientale extract to the treatment of nonalcoholic fatty liver disease in clinics.
Key words: nonalcoholic fatty liver disease (NAFLD), Alisma orientale, lipoapoptosis, HepG2 cell, free fatty acids (FFAs)
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