Anti-cancer Effects of <i>Oldenlandia diffusa, Cremastra appendiculata</i> and
<i>Fritillaria thunbergii</i> on MDA-MB-231 Human Breast Cancer Cells

Jin, Myung-ho; Park, Sun-young; Kang, You-gyung; Shim, Won-suk; Hur, Hee-soo; Hong, Sang-hoon; Park, Cheol; Choi, Yung-hyun; Park, Sang-eun

The Journal of Internal Korean Medicine > Volume 35(2); 2014 > Article

Original Article

The Journal of Internal Korean Medicine 2014;35(2): 133-144.

白花蛇舌草, 山慈姑, 浙貝母에 의한 MDA-MB-231 인체 유방암 세포에서의 항암 효과

진명호¹, 박선영¹, 강유경¹, 심원석¹, 허희수¹, 홍상훈¹, 박철², 최영현³, 박상은¹

¹동의대학교 한의과대학 한방내과
²동의대학교 분자생물학과
³동의대학교 한의과대학 생화학교실

Anti-cancer Effects of Oldenlandia diffusa, Cremastra appendiculata and Fritillaria thunbergii on MDA-MB-231 Human Breast Cancer Cells

Myung-ho Jin¹, Sun-young Park¹, You-gyung Kang¹, Won-suk Shim¹, Hee-soo Hur¹, Sang-hoon Hong¹, Cheol Park², Yung-hyun Choi³, Sang-eun Park¹

¹Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University
²Dept. of Molecular Biology, Dong-Eui University
³Dept. of Biochemistry, College of Korean Medicine, Dong-Eui University

Correspondence

Sang-eun Park ,Tel: 052-226-8106, Fax: 052-256-0665, Email: hanseul@hanmail.net

Published online: June 30, 2014.

ABSTRACT

O. diffusa, C. appendiculata and F. thunbergii are reported to possess many pharmacological activities including anti-oxidant, anti-inflammatory, anti-hypertension, anti-diabetic and anti-cancer effects. However, their anti-cancer activities in human breast cancer have not been clearly elucidated yet. Objectives:
In the present study, we compared the in vitro cytotoxic effects of single and complex treatment of O. diffusa, C. appendiculata and F. thunbergii in human breast cancer MDA-MB-231 cells.

Methods:
After we treated human breast cancer MDA-MB-231 cells with O. diffusa, C. appendiculata and F. thunbergii. we evaluated viability, growth inhibition, morphological changes, apoptotic body formation, measurement of the cell cycle and formation of DNA fragmentation of these cells.

Results:
We found that single treatment of O. diffusa and F. thunbergii could inhibit cell proliferation in human breast cancer MDA-MB-231 cells. However, complex treatment of O. diffusa, C. appendiculata and F. thunbergii had weak or no effect on the cell proliferation of MDA-MB-231 cells. The first, anti-proliferative effects of O. diffusa in MDA-MB-231 cells was associated with G2/M arrest of cell cycle and apoptotic cell death. The second, anti-proliferative effect of F. thunbergii
Conclusions:
Taken together, these findings suggest that O. diffusa and F. thunbergii may be a potential chemotherapeutic agent for the control of human breast cancer cells, further studies will be needed to identify the molecular mechanisms.

Key words: O. diffusa, C. appendiculata, F. thunbergii, human breast cancer cells, apoptosis