| 신경아세포종에 대한 八寶廻春湯의 항암 효과 |
| 안정환1, 조문영2, 우찬2, 신용진2, 신선호2 |
1약천한의원
2원광대학교 한의과대학 내과학교실 |
| Anti-cancer Effects of Palbohoichoon-tang on Neuroblastoma Cells |
| Jung-hwan An1, Mun-young Cho2, Chan Woo2, Yong-jin Shin2, Sun-ho Shin2 |
1YacCheon Oriental Medical Clinic
2Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University |
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Published online: March 30, 2014. |
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| ABSTRACT |
Objectives:
To investigate the anti-cancer effect of Palbohoichoon-tang (PBHCT) extracts.
Methods :
The cell viability was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MMT) assay and cell morphological changes were microscopically analyzed after staining with 10 μM 2-[4-amidinophenyl]-6-indolecarbamidine dihydrochloride (DAPI) and TUNEL. We also analyzed expression of Bcl2, BclxL, Bax, procaspase-3, procaspase-9, and procyclic acidic repetitive protein (PARP) by western blot method.
Results:
Observations showed that PBHCT induced the apoptotic cell death proved by increased sub-G1 phase cell population, apoptotic body formation and chromatin condensation. Western blot analysis of total cell lysates revealed that the PBHCT induced cleavage of caspase-9, caspase-3 and poly (ADP-ribose) polymerase (PARP). In addition, PBHCT dose-dependently increased the activity of caspase-9, caspase-3 and PARP-1. Furthermore, PBHCT reduced anti-apoptotic Bcl2, BclxL expression which contributed to the loss of mitochondrial membrane potential and the activations of caspase-9 and caspase-3.
Conclusions:
These findings suggest that PBHCT exerts anti-cancer effects on human neuroblastoma SH-SY5Y cells by inducing apoptotic death via down-regulation of anti-apoptotic proteins such as Bcl2 and BclxL, up-regulation of pro-apoptotic proteins such as Bax, and activation of caspase cascades and PARP-1. |
| Key words:
SH-SY5Y cell, Anti-cancer effect, Palbohoichoon-tang (PBHCT) |
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