A Comparative Study of <i>Eejin-tang, Hyangsaeejin-tang</i> and <i>Naeso-san</i> Extracts on
Indomethacin-induced Gastric Mucosal Lesions in Mice

Park, Jung-han; Baik, Tai-hyeun

The Journal of Internal Korean Medicine > Volume 34(4); 2013 > Article

Original Article

The Journal of Internal Korean Medicine 2013;34(4): 412-427.

Indomethacin으로 유발된 생쥐의 위점막 손상에 대한 二陳湯, 香砂二陳湯 및 內消散의 비교연구

박정한, 백태현

상지대학교 한의과대학 비계내과학교실

A Comparative Study of Eejin-tang, Hyangsaeejin-tang and Naeso-san Extracts on Indomethacin-induced Gastric Mucosal Lesions in Mice

Jung-han Park, Tai-hyeun Baik

Dept. of Internal Medicine, College of Korean Medicine, Sang-Ji University

Correspondence

Tai-hyeun Baik ,Tel: 033-741-9207, Email: white@sangji.ac.kr

Published online: December 30, 2013.

ABSTRACT

Objectives:
This study was carried out to compare the effects of Eejin-tang, Hyangsaeejin-tang and Naeso-san extracts on indomethacin-induced gastric mucosal lesions in mice.

Methods :
Experimental mice were divided into six groups. The normal group had no gastro-inflammation. In the control group, gastro-inflammation was elicited by indomethacin. Misoprostol, Eejin-tang, Hyangsaeejin-tang and Naeso-san group were those in which misoprostol, Eejin-tang extract, Hyangsaeejin-tang extract and Naeso-san extract were administered after gastro-inflammation is elicited. This study examined the anti-inflammation effects and distribution of mucus secreting cells, zonula occludin-1 (ZO-1), heat shock protein (HSP) 70, periodic acid-schiff reaction stain (PAS), peanut agglutinin (PNA), cyclooxygenase-1 (COX-1), 5-bromo-2‘-deoxyuridine (BrdU), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2).

Results:
1. The hemorrhagic erosion and damaged mucus secreting cell, the positive reaction HSP70 increased in the control group, but decreased in the Eejin-tang, Hyangsaeejin-tang and Naeso-san groups. 2. The positive reaction of ZO-1, PAS, PNA, COX-1 and BrdU decreased in the control group, but increased in the Eejin-tang, Hyangsaeejin-tang and Naeso-san groups. 3. The positive reaction of NF-kB p65, iNOS and COX-2 increased in the control group, but decreased in the Eejin-tang, Hyangsaeejin-tang and Naeso-san groups.

Conclusions:
Among the three extracts, the effects were in the order of Naeso-san, Hyangsaeejin-tang and Eejin-tang group, Naeso-san being the most effective.

Key words: Eejin-tang (Erchen-tang), Hyangsaeejin-tang (Xiangshaerchen-tang), Naeso-san (Neixaio-san), indomethacin, gastric mucosal lesions