| 茵蔯의 에탄올 추출물이 담도암 세포주 SNU-1196의 apoptosis에 미치는 효과에 관한 연구 |
| 이경욱, 우홍정 |
| 경희대학교 한의과대학 간계내과학교실 |
| Study on the Pro-apoptotic Effect of Artemisiae Capillaris Herba Extracted
with Ethanol on Biliary Tract Cancer Cell Line, SNU-1196 |
| Kyung-wook Lee, Hong-jung Woo |
| Dept. of Internal Medicine, College of Korean Medicine, Kyung-Hee University |
| Correspondence |
Hong-jung Woo ,Tel: 02-958-9118, Fax: 02-958-9120, Email: hjwoo@khu.ac.kr
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Published online: December 30, 2012. |
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| ABSTRACT |
Objectives:
This study was performed to elucidate the pro-apoptotic effect of Artemisiae capillaris herba extracted with ethanol on biliary tract cancer cells.
Materials and Methods :
The biliary tract cancer cell line SNU-1196 was used in this study. Cells were treated with different concentrations of Artemisiae capillaris herba for 24, 48 and 72 hours. After the treatment, cell viability, apoptosis, caspase activities and the mRNA expressions of the Bcl-2, Bax, P53, and P21 were measured by using MTT assay, cell cycle analysis, apoptosis assay, and RT-PCR. The cell cycle analysis was done by flow cytometry and apoptosis assay by cell death detection ELISA kit.
Results:
Artemisiae capillaris herba inhibited proliferation of SNU-1196 in long-time culture group with dose-dependent manner. All cells treated with Artemisiae capillaris herba showed increased apoptosis with dose- and time-dependent manner. Exposure of SNU-1196 to Artemisiae capillaris herba induced caspase-3 activation. However, apeoptosis was blocked when SNU-1196 was treated together with the pan-caspase inhibitor Z-VAD-FMK and the caspase-3 inhibitor Z-DEVE-FMK. After the treatment of Artemisiae capillaris herba, the mRNA expressions of caspase -3, -8, -9, p53, and p21 was increased in all cells. Artemisiae capillaris herba resulted in a significant decrease in Bcl-2 and an increase in Bax mRNA levels.
Conclusions:
These results suggest that Artemisiae capillaris herba would be beneficial in the treatment of biliary tract |
| Key words:
Artemisiae capillaris herba, biliary tract cancer, apoptosis |
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