Anticancer and Related Immunomodulatory Effects of <i>Kwibi-tang</i> on Non-small Cell
Lung Carcinoma, NCI-H520, Xenograft Mice

Son, Ji-young; Choi, Hae-yun; Kim, Jong-dae

The Journal of Internal Korean Medicine > Volume 33(4); 2012 > Article

Original Article

The Journal of Internal Korean Medicine 2012;33(4): 387-404.

歸脾湯이 비소세포 폐암세포(NCI-H520) 이식 마우스에서 항암 및 면역 활성에 미치는 영향

손지영, 최해윤, 김종대

대구한의대학교 한의과대학 내과학교실

Anticancer and Related Immunomodulatory Effects of Kwibi-tang on Non-small Cell Lung Carcinoma, NCI-H520, Xenograft Mice

Ji-young Son, Hae-yun Choi, Jong-dae Kim

Dept. of Internal Medicine, College of Oriental Medicine, Daegu Haany University

Correspondence

Jong-dae Kim ,Tel: 053-770-2122, Fax: 053-770-2189, Email: omendoim@dhu.ac.kr

Published online: December 30, 2012.

ABSTRACT

Objectives:
This study was to observe anticancer and related immunomodulatory effects of Kwibi-tang extracts (KBTe) on non-small cell lung carcinoma (squamous epithelial carcinoma), NCI-H520, xenograft Balb/c nu-nu nude mice.

Methods :
Three different dosages of KBTe, 50, 100 and 200 mg/kg were orally administered once a day for 42 days from 11 days after tumor cell inoculation. Six groups, each of 8 mice per group were used in the present study. Changes in body weight, tumor volume and weight, lymphatic organs (spleen and popliteal lymph node), serum interferon (IFN)-γ levels, splenocytes NK cell activity and peritoneal macrophage activities, splenic tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-10 contents were observed with tumor mass and lymphatic organ histopathology to detect anticancer and immunomodulatory effects. The results were compared with a potent cytotoxic anticancer agent, 5-FU (5-Fluorouracil) 30 mg/kg, intraperitoneal treatment (3-day intervals for 42 days, the optimal effective treatment regimes already confirmed).

Results & Conclusions :
This study suggest that over 50 mg/kg of KBTe showed favorable anticancer effects on the NCI-H520 cell xenograft with immunomodulatory effects. Although relatively lower anticancer effects were observed in KBTe 200 mg/kg treated mice as compared with 5-FU 30 mg/kg treated mice, no meaningful favorable immunomodulatory effects were observed after 5-FU treatment in the present study.

Key words: Kwibi-tang (Guipi-tang) extracts, anticancer effects, immunomodulatory effects, non-small cell lung carcinoma, Balb/c nu-nu nude mice