Microarray Analysis of Gene Expression Profile by Treatment of Schizandrae fructus
Extract in Inflammation-induced Human Epithelial A549 Cells

Jung, Jin-yong; Jung, Sung-ki; Jung, Hee-jae; Rhee, Hyung-koo

The Journal of Internal Korean Medicine > Volume 29(3); 2008 > Article

Original Article

The Journal of Internal Korean Medicine 2008;29(3): 543-553.

염증이 유발된 인간기관지상피세포에서 오미자가 Microarray를 이용한 유전자 발현 분석에 미치는 영향

정진용, 정승기, 정희재, 이형구

경희대학교 한의과대학 폐계내과학 교실

Microarray Analysis of Gene Expression Profile by Treatment of Schizandrae fructus Extract in Inflammation-induced Human Epithelial A549 Cells

Jin-yong Jung, Sung-ki Jung, Hee-jae Jung, Hyung-koo Rhee

Division of Allergy, Immune & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University

Correspondence

Hyung-koo Rhee ,Tel: 02-958-9147, Fax: 02-958-9148, Email: rheehk@khu.ac.kr

Published online: September 30, 2008.

ABSTRACT

Objective :
The goal of this study was to determine the anti-asthma mechanism of SF on TNF-α induced activation on A549 (human typeⅡ-like epithelial) cells. Using oligonucleotide microarray, we sought to establish the molecular mechanism of the protective effects of SF on A549 cells. Material &

Methods :
Cells were cultured in three different conditions: 1) negative control group was cultured in normal condition of DMEM, 2) positive control group was activated with TNF-α, IL-4, and IL-1β, and 3) SF treated group was previously treated with 0.1㎍/㎖ SF after TNF-α, IL-4, and IL-1 activation. Cells of positive control and SF treated groups were cultured for 30 min, 1hr, 3hr and 6hr.

Results:
The comparative analysis of the gene expression profile revealed that proinflammatory cytokines such as IL1F8, IL1F9, IL1R1, IL1RN, IL1RAPL1, IL8, TNFRSF4, TNFSF10c, TNFSF13, TRAF5, and TRAF7 and inflammation-related genes including MMP2, MMP11, MMP14, MMP15, MMP16, MMP19, MMP25, and MMP27 were down regulated with SF treatment. Cell adhesion molecule genes such as ITGB1, ITGBL1, selectin P ligand, selectin E, ICAM2, ICAM3, VCAM1, PECAM, FCER1G and MMP28 genes were also down-regulated in SF treated A549 cells.

Conclusion:
These results suggest that the anti-asthmatic effects of SF could be mediated by regulating specific genes related with cell adhesion, proinflammatory cytokine and inflammation-related genes in A549 cells.

Key words: TNF-α, Schizandrae fructus, Gene expression profile, Microarray