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The Journal of Internal Korean Medicine 2008;29(3): 543-553. |
염증이 유발된 인간기관지상피세포에서 오미자가 Microarray를 이용한 유전자 발현 분석에 미치는 영향 |
정진용, 정승기, 정희재, 이형구 |
경희대학교 한의과대학 폐계내과학 교실 |
Microarray Analysis of Gene Expression Profile by Treatment of Schizandrae fructus
Extract in Inflammation-induced Human Epithelial A549 Cells |
Jin-yong Jung, Sung-ki Jung, Hee-jae Jung, Hyung-koo Rhee |
Division of Allergy, Immune & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University |
Correspondence |
Hyung-koo Rhee ,Tel: 02-958-9147, Fax: 02-958-9148, Email: rheehk@khu.ac.kr
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Published online: September 30, 2008. |
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ABSTRACT |
Objective : The goal of this study was to determine the anti-asthma mechanism of SF on TNF-α induced activation on A549 (human typeⅡ-like epithelial) cells. Using oligonucleotide microarray, we sought to establish the molecular mechanism of the protective effects of SF on A549 cells. Material &
Methods : Cells were cultured in three different conditions: 1) negative control group was cultured in normal condition of DMEM, 2) positive control group was activated with TNF-α, IL-4, and IL-1β, and 3) SF treated group was previously treated with 0.1㎍/㎖ SF after TNF-α, IL-4, and IL-1 activation. Cells of positive control and SF treated groups were cultured for 30 min, 1hr, 3hr and 6hr.
Results: The comparative analysis of the gene expression profile revealed that proinflammatory cytokines such as IL1F8, IL1F9, IL1R1, IL1RN, IL1RAPL1, IL8, TNFRSF4, TNFSF10c, TNFSF13, TRAF5, and TRAF7 and inflammation-related genes including MMP2, MMP11, MMP14, MMP15, MMP16, MMP19, MMP25, and MMP27 were down regulated with SF treatment. Cell adhesion molecule genes such as ITGB1, ITGBL1, selectin P ligand, selectin E, ICAM2, ICAM3, VCAM1, PECAM, FCER1G and MMP28 genes were also down-regulated in SF treated A549 cells.
Conclusion: These results suggest that the anti-asthmatic effects of SF could be mediated by regulating specific genes related with cell adhesion, proinflammatory cytokine and inflammation-related genes in A549 cells. |
Key words:
TNF-α, Schizandrae fructus, Gene expression profile, Microarray |
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