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The Journal of Internal Korean Medicine > Volume 29(2); 2008 > Article
The Journal of Internal Korean Medicine 2008;29(2): 500-511.
生肝健脾湯加三七根이 흰쥐의 간섬유화 억제에 미치는 영향
이은1, 김영철2, 고흥1
1세명대학교 내과학교실
2경희대학교 내과학교실
Inhibitory Effect of Saengangeonbitang-gasamchilgn on Liver Fibrosis in Rat
Eun Lee1, Young-chul Kim2, Heung Ko1
1Department of internal Medicine, College of Oriental Medicine, Semyung University
2Department of internal Medicine, College of Oriental Medicine, Kyunghee University
Correspondence  Heung Ko ,Tel: 043-649-1813, Email: yurie99@hanmail.net
  Published online: June 30, 2008.
ABSTRACT
The aim of this study was to investigate the inhibitory effect of Saengangeonbitang-gasamchilgn(SGGBTGSCG) on collagen production in rat hepatic stellate cells(HSC) and on the TAA-induced chronic liver injury model in rats.

Methods :
1) HSCs were treated with SGGBTGSCG extract powder(50% EtOH SGGBTGSCG, dw SGGBTGSCG). After the treatment, MTT assay, BrdU assay and procollagen assay were done. In addition, gene expressions of collagen type 1 α2, ASMA, TIMP1, and TIMP2, all of which are known to be associated with liver fibrosis, were analyzed by RT-PCR. 2) Liver fibrosis was developed in rats by injection of TAA 3 times a week for 5 weeks. After the SGGBTGSCG-treatment, body weight, liver & spleen weight, liver function test, the complete blood cell count and the change of portal pressure were studied.

Results:
In MTT assay, SGGBTGSCG significantly decreased the viability of HSCs in a dose-dependent manner. In BrdU assay, SGGBTGSCG significantly inhibited the HSC proliferation in a dose-dependant manner. In procollagen assay, SGGBTGSCG decreased procollagen production by HSC. In the change of rats' liver and spleen weight, TAA+SGGBTGSCG groups showed little difference compared with TAA-only group. In the liver function test, SGGBTGSCG decreased the serum level of ALT, AST, and Alp elevated by TAA. In the complete blood cell count, SGGBTGSCG significantly decreased WBC elevated by TAA and increased RBC and Hct lowered by TAA. In the change of portal pressure, SGGBTGSCG decreased portal pressure elevated by TAA.

Conclusions:
These results suggest that SGGBTGSCG is beneficial in the treatment of cirrhotic patients as well as for patients with chronic hepatitis.
Key words: Saengangeonbitang(SGGBT), hepatic stellate cells(HSCs), liver fibrosis, collagen, RT-PCR.
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