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The Journal of Internal Korean Medicine > Volume 29(2); 2008 > Article
The Journal of Internal Korean Medicine 2008;29(2): 421-431.
楡根皮 추출물의 활성종 억제 및 염증 촉진 인자 제어 효과
조은영, 정지천
동국대학교 한방병원 내과
The Inhibitory Effects of Ulmus davidiana on the Reactive Species and Proinflammatory Proteins
Eun-young Jo, Ji-cheon Jeong
Department of Oriental Internal Medicine, Hospital of Oriental Medicine, Dongguk University
Correspondence  Eun-young Jo ,Tel: 054-770-1342, Fax: 054-770-1500, Email: party94@hanmail.net
  Published online: June 30, 2008.
This study was to investigate the inhibitory effects of Ulmus davidiana on the generation of peroxynitrite (ONOO-), nitric oxide (NO) and superoxide anion radicals (․O2 -) in the endothelial cells of rat vessels. The effects of Ulmus davidiana on the expression of inflammation-related proteins, NF-κB (p50, p65), COX-2, and iNOS, were examined by western blotting.

Methods :
For this study, fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed via using anti-NF-κB (p50, p65), anti-COX-2, and anti-iNOS, respectively.

Ulmus davidiana inhibited the generation of ONOO-, NO and ․O2 - in the lipopolysaccharide (LPS)-treated endothelial cells of rat vessels in vitro. Ulmus davidiana inhibited the expression of COX-2 and iNOS genes by means of decreasing the NF-κB activation.

These results suggest Ulmus davidiana is effective on inhibiting the generation of ONOO-, NO and ․O2 -, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases.
Key words: Ulmus davidiana, peroxynitrite, nitric oxide, superoxide anion radical, NF-κB.
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