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The Journal of Internal Korean Medicine > Volume 29(2); 2008 > Article
The Journal of Internal Korean Medicine 2008;29(2): 334-347.
解熱湯이 LPS로 자극된 대식세포에 있어 염증관련 Cytokine 발현억제에 미치는 효과
이병삼1, 김홍렬2, 김진주3, 정승기1, 이형구1, 정희재1
1경희대학교 한의과대학 폐계내과학교실
2경희대학교 한의과대학 생화학교실
3경희대학교 약학대학 한방생리학교실
The Inhibitory Effects of Haeyeol-tang in Expression of Pro-inflammatory Cytokine on LPS-stimulated THP-1 Cells
Byeong-sam Lee1, Hong-yeoul Kim2, Jin-ju Kim3, Sung-ki Jung1, Hyung-koo Rhee1, Hee-jae Jung1
1Division of Allergy, Immune & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University
2Department of Biochemistry college of Oriental Medicine, Kyung Hee University
3Department of Orientalphysiology college of pharmacy Kyung Hee University
Correspondence  Hee-jae Jung ,Tel: 02-958-9147, Fax: 02-958-9148, Email: jskes@unitel.co.kr
  Published online: June 30, 2008.
ABSTRACT
Background and Objective :
Haeyeol-tang, composed of Houttuyniae Herba, Lonicerae Flos, Taraxaci Herba, and Scrophulariae Radix, is widely used for alleviating the symptom of various kinds of inflammatory pulmonary disease, including asthma and COPD. We want to know whether Haeyeol-tang has an anti-inflammatory effect by analyzing expression of pro-inflammatory cytokines.

Materials and Methods :
We differentiated the THP-1 cells into macrophage-like cells by treatment with PMA. Inflammation was induced by treatment with LPS and PMA. We found the safe concentration of Haeyeol-tang by using MTS assay and used PD98059 as a negative control for comparison of anti-inflammatory effect of Haeyeol-tang.

Results:
The RT-PCR analysis results show that the cell survival rate is over 100% within 1 ng/mL to 1 ug/mL of Haeyeol-tang and begins to decrease under 100% at 10 ug/mL. The gene expression of IL-1β, IL-6, IL-8, IL-10, TNF-α and TGF-β levels were down-regulated when Haeyeol-tang was treated at concentrations between 1 ng/mL an 1 ug/mL on monocyte-derived macrophages. Interestingly, 1 ug/mL Haeyeol-tang-treated samples showed that the transcriptional activities of IL-8, TNF-α, IL-10 and TGF-β were more down-regulated than those of PD098059 (TNF-α inhibitor). At protein level, the ELISA analysis results showed that there were more remarkable (p<0.001) decreases in expression of IL-1β, IL-6, IL-8 and TNF-α on both the 1 ug/mL Haeyeol-tang-treated group and the PD98059-treated group than the LPS-treated group.

Conclusion:
We conclude that Haeyeol-tang has an anti-inflammatory effect by inhibiting expression of pro-inflammatory cytokines and chemokines at mRNA and protein levels. These results may provide us a promising way to care for general inflammatory diseases as well as inflammatory pulmonary disease, including asthma and COPD, with further clinical study.
Key words: Haeyeol-tang, IL-1β, IL-6, IL-8, IL-10, TNF-α, TGF-β
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