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The Journal of Internal Korean Medicine > Volume 29(1); 2008 > Article
The Journal of Internal Korean Medicine 2008;29(1): 130-148.
A539 및 NCI-H460 인체 폐암세포의 증식 및 apoptosis 유도에 미치는 가미삼기보폐탕의 영향
김진영1, 김현중1, 정광식1, 박철2, 최영현2, 감철우1, 박동일1
1동의대학교 한의과대학 내과학교실
2동의대학교 한의과대학 생화학교실
Effect of Gamisamgibopae-tang on the Growth and Apoptosis of A539 and NCI-H460 Human Lung Cancer Cells
Jin-young Kim1, Hyun-joong Kim1, Kwang-sik Jung1, Cheol Park2, Yung-hyun Choi2, Cheol-woo Kam1, Dong-il Park1
1Departments of Internal Medicine, Dongeui University College of Oriental Medicine
2Departments of Biochemistry, Dongeui University College of Oriental Medicine
Correspondence  Dong-il Park ,Tel: 051-850-8650, Fax: 051-867-5162, Email: dipark@deu.ac.kr
  Published online: March 30, 2008.
Objective :
This study was designed to investigate the effect of the water extract of Gamisamgibopae-tang(GMSGBPT), an oriental herbal formulation, on the growth of NCI-H460 and A549 human non-small-cell lung cancer cell lines.

Methods :
Cytotoxicity and cell morphology were evaluated by MTT assay and inverted microscope, respectively. Apoptosis was detected using agarose gel electrophoresis and flow cytometer. The expression levels of mRNAs and proteins of target genes were determined by RT-PCR and western blot analyses, respectively.

Result and Conclusion :
We found that exposure of A549 cells to GMSGBPT resulted in the growth inhibition in a dose-dependent manner as measured by MTT assay, but GMSGBPTdid not affect the growth of NCI-H460 cells. The anti-proliferative effect of GMSGBPT treatment in A549 cells was associated with morphological changes, formation of apoptotic bodies and DNA fragmentation, and flow cytometry analysis confirmed that GMSGBPT treatment increased the populations of apoptotic-sub G1 phase. Growth inhibition and apoptotic cell death by GMSGBPT were connected with a up-regulation of cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) mRNA and protein in a tumor suppressor p53-independent fashion. However GMSGBPT treatment did not affect other growth regulation-related genes such as early growth response-1 (Egr-1), nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1), inducible nitric oxide synthase (iNOS), cyclooxygenases (COXs), telomere-regulatory factors in A549 orNCI-H460 cells. Taken together, these findings partially provide novel insights into the possible molecular mechanism of the anti-cancer activity of GMSGBPT.
Key words: Gamisamgibopae-tang, non-small-cell lung cancer cells, NCI-H460, A549, apoptosis
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