Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 87 Warning: chmod() expects exactly 2 parameters, 3 given in /home/virtual/lib/view_data.php on line 88 Warning: fopen(/home/virtual/jikm/journal/upload/ip_log/ip_log_2024-07.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 95 Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 96 Inhibition of Cell Proliferation and Induction of Apoptosis by Methanolic Extract of Aconiti Tuber in Human Renal Cell Carcinoma Cells
| Home | E-Submission | Sitemap | Editorial Office |  
The Journal of Internal Korean Medicine > Volume 29(1); 2008 > Article
The Journal of Internal Korean Medicine 2008;29(1): 42-66.
부자의 메탄올 추출물에 의한 신세포암의 증식억제 및 세포사멸 유도
김종환1, 최영현2, 황원덕1
1동의대학교 한의과대학 신계내과학교실
2동의대학교 한의과대학 생화학교실
Inhibition of Cell Proliferation and Induction of Apoptosis by Methanolic Extract of Aconiti Tuber in Human Renal Cell Carcinoma Cells
Jong-hwan Kim1, Yung-hyun Choi2, Won-deuk Hwang1
1Department of Oriental Medicine, Dongeui University
Correspondence  Won-deuk Hwang ,Tel: 051-850-8625, Fax: 051-867-5162, Email: hwooso@hanafos.com
  Published online: March 30, 2008.
Aconiti Tuber is a traditional medicinal plant generally used in Oriental medicine therapy. In this study, we investigated the biochemical mechanisms of anti-proliferative effects by the methanol extract of Aconiti tuber (MEBJ) in Caki-1 human renal cell carcinoma cells. It was found that MEBJ could inhibit, in a dose-dependent manner, cell growth which was associated with apoptotic cell death such as formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase. Apoptosis of Caki-1 cells by MEBJ was associated with an up-regulation of pro-apoptotic Bax expression, and a down-regulation of anti-apoptotic Bcl-2 in a dose-dependent manner; however, the levels of IAP family were not affected. MEBJ treatment also induced the proteolytic activation of caspase-3 and -8, and a inhibition of poly(ADP-ribose) polymerase (PARP) and PLCγ1 protein. Furthermore, MEBJ treatment caused a dose-dependent inhibition of iNOS and cyclooxygenase-2 (Cox-2). Though further studies will be needed to identify the active compounds that confer the anti-cancer activity of MEBJ, the present findings provide important new insights into the possible molecular mechanisms of the apoptotic activity of MEBJ in cancer cells.
Key words: Aconiti tuber, Renal cell carcinoma, Apoptosis, Bax protein, Death receptors.
Editorial Office
Dongguk University Ilsan Oriental Hospital
27, Dongguk-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326 Korea
TEL: +82-31-961-9046   FAX: +82-31-961-9049   E-mail: jikm.edit@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © The Society of Internal Korean Medicine.                 Developed in M2PI      |      Admin Login