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The Journal of Internal Korean Medicine > Volume 29(1); 2008 > Article
The Journal of Internal Korean Medicine 2008;29(1): 32-41.
한국인 천식환자의 Monocyte chemoattractant protein 1(MCP-1) 유전자 다형성에 대한 분석
황우석1, 정승연1, 김진주2, 정희재1, 정승기1
1경희대학교 한의과대학 폐계내과학 교실
2경희대학교 약학대학 한방생리학교실
Analysis of Monocyte Chemoattractant Protein 1(MCP-1) Polymorphism in Korean Patients with Asthma
Woo-suk Hwang1, Seung-yeon Jeong1, Jin-ju Kim2, Hee-jae Jung1, Sung-ki Jung1
1Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University
2Dept. of Oriental Physiology, College of Pharmacy, Kyung-Hee University
Correspondence  Sung-ki Jung ,Tel: 02-958-9147, Fax: 02-958-9148, Email: jskes@unitel.co.kr
  Published online: March 30, 2008.
ABSTRACT
Background:
Monocyte chemoattractant protein-1(MCP-1), one of the CC chemokines, appears to play a significant role in asthma pathogenesis. It was reported that polymorphism in the MCP-1(-2518 A/G promoter) was associated with asthma in Caucasians, but the association of this polymorphism and asthma patients in the Korean population has not yet been clarified.

Objective :
We investigated the possible association between 2 polymorphisms (-2518 A/G promoter and Cys35Cys) and asthma patients in a Korean population.

Materials and Methods :
DNA samples were obtained from 86 Korean asthma patients and 270 healthy controls. MCP-1 genomic variants (-2518 A/G promoter and Cys35Cys polymorphism) were detected by PCR-RFLP. Level of MCP-1 was measured by ELISA for each genotype (n=8) (AA, AG, GG) and allele types of -2518 A/G promoter polymorphism for control subjects.

Results:
The Cys35Cys polymorphism was associated with asthma patients in Korean population [genotype distribution (χ²=16.011, P<0.001)]. Comparison of the two groups revealed no detectable differences in genotype and allele frequencies of the -2518 A/G polymorphism. Haplotype frequencies analysis revealed significant difference (χ²=51.70, P<0.001). MCP-1 serum level of subjects with G genotype of -2518 A/G promoter polymorphism was statistically higher than that with AA genotype (P<0.05).

Conclusion:
Our data indicate that no association exists between the MCP-1 -2518 A/G polymorphism and asthma susceptibility in the Korean population. However, it is noteworthy that the high prevalence of the -2518 G allele in the Korean population suggests a potentially important ethnic variation in the regulation of MCP-1 production. This variation must be considered in gene-association studies in different ethnic populations.
Key words: Asthma, MCP-1, -2518 A/G, Cys35Cys, Polymorphism.
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