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The Journal of Internal Korean Medicine 2007;28(4): 681-693. |
| 蒼朮이 천식 관련 Th1/Th2 세포 분비 cytokine에 미치는 영향 |
| 이정우, 이형구, 정희재 |
| 경희대학교 한의과대학 폐계내과학 교실 |
| Studying of the Effects of Atractylodes Japonica Extract on Th1/Th2 Cell-derived Cytokines |
| Jeong-woo Lee, Hyung-koo Rhee, Hee-jae Jung |
| Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung Hee University |
| Correspondence |
Hee-jae Jung ,Tel: 02-958-9147, Fax: 02-958-9148, Email: hanfish@khmc.or.kr
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Published online: December 30, 2007. |
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| ABSTRACT |
Background and Objective :
Atractylodes japonica (AJ) is a commonly-used herbal medicine in Asian countries such as Korea, China and Japan. The present study was designated to evaluate the direct effects of AJ on helper T cell activities and on Th1/Th2 lineage development in vitro.
Materials and Methods :
Spleen cells from 8-week BALB/c mice were cultured in CR extracts containing medium without activation for 24 hours and with activation for 48 hours. CD4+ T cells were isolated and analyzed for mRNA expression levels of INF-γ, IL-4, T-bet and GATA-3 by RT-PCR and secretion cytokines levels of INF-γ, IL-2, IL-4, IL-5 and IL-10 by ELISA.
Results:
The results demonstrated that AJ had no mitogenic effects on unstimulated CD4+ T cells, but augmented CD4+T-cell proliferation upon activation with anti-CD3/anti-CD28 antibodies in a dose-dependent manner. AJ treatment significantly increased CD4+ T cell population and IFN-γ expression was significantly enhanced, while IL-4 expression significantly decreased. In addition, in vitro Th1/Th2 polarization experiments revealed that AJ enhanced IFN-γ secretion in Th1 cells, but reduced the IL-4 in Th2 cells in dose-dependent manner.
Conclusion:
These results suggest that AJ treatment could be a desirable alternative therapy for the prevention or correction of Th2 dominant pathological disorders, such as allergy and asthma. |
| Key words:
Atractylodes japonica (AJ) , asthma, INF-γ, IL-4, T-bet, GATA-3, IL-5, IL-10 |
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