<i>Shigyungbanha-tang</i> Exhibits Anti-inflammatory Effects by Inhibiting IκB-α Degradation
in LPS-stimulated Peritoneal Macrophages

Lee, Seung-eon; Shin, Jo-young; Lee, Si-hyeong

The Journal of Internal Korean Medicine > Volume 28(3); 2007 > Article

Original Article

The Journal of Internal Korean Medicine 2007;28(3): 442-452.

LPS로 유도한 복강대식세포에서 IκB-α 분억제에 의한 柴梗半夏湯의 항염증효과

이승언¹, 신조영¹, 이시형²

¹원광대학교 한의과대학 폐계내과학교실
²원광대학교 한의학전문대학원

Shigyungbanha-tang Exhibits Anti-inflammatory Effects by Inhibiting IκB-α Degradation in LPS-stimulated Peritoneal Macrophages

Seung-eon Lee¹, Jo-young Shin¹, Si-hyeong Lee²

¹Department of Internal Medicine, College of Oriental Medicine Wonkwang University
²Department of Professional Graduate School of Oriental Medicine Wonkwang University

Correspondence

Seung-eon Lee ,Tel: 051-532-8300, Email: seyi93@hanmail.net

Published online: September 30, 2007.

ABSTRACT

Objectives:
The purpose of this study was to investigate the toll-like receptor (TLR)-4 mediated anti-inflammatory effects of extract from Shigyungbanha-tang (SBT) on the peritoneal macrophage.

Methods :
To evaluate of TLR-4 mediated inflammatory of SBT, we examined NO and cytokine production in TRL-4 ligand (LPS : lipopolysaccharide) induced macrophages. Furthermore, we examined its molecular mechanism using western blot.

Results:
Extract from SBT itself does not have any cytotoxic effect in the peritoneal macrophages. Extract from SBT reduced LPS-induced nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6 and IL-12 production in peritoneal macrophages. SBT inhibited degradation of inhibitor kappa B-alpha (IκB-α) in the TLR-4 mediated peritoneal macrophages.

Conclusions:
These results suggest that SBT inhibits NO and cytokines production through inhibiting nuclear factor-kappaB (NF-κB) activation in peritoneal macrophage and that SBT may be beneficial oriental medicine for inflammation

Key words: Shigyungbanha-Tang, IκB-α, NF-κB, Lipopolysaccharide(LPS)