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The Journal of Internal Korean Medicine 2007;28(1): 149-165. |
| 茵蔯, 鬱金, 桑寄生, 瓦松이 HepG2 cell의 혈관생성인자 발현에 미치는 영향 |
| 김지권, 김영철, 이장훈, 우홍정 |
| 경희대학교 한의과대학 간계내과학교실 |
| The Effects of Artermisiae Capillaris Herba, Curcumae Rhizoma, Loranthi Ramulus, and Orostachys Herba on Expression of Angiogenic Factors in HepG2 Cells |
| Ji-kwon Kim, Young-chul Kim, Jang-hoon Lee, Hong-jung Woo |
| Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University |
| Correspondence |
Hong-jung Woo ,Tel: 02-958-9118, Fax: 02-958-9120, Email: hjwoo@khu.ac.kr
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Published online: March 30, 2007. |
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| ABSTRACT |
Objectives:
This study was designed to investigate the effects of Artermisiae Capillaris Herba, Curcumae Rhizoma, Loranthi Ramulus, and Orostachys Herba on expression of angiogenic factors in HepG2 cells.
Materials and Methods :
The mRNA expression level and protein secretion level of angiogenic factors were measured using quantitative RT-PCR, western blot and ELISA assay respectively in Artermisiae Capillaris Herba, Curcumae Rhizoma, Loranthi Ramulus, Orostachys Herba -treated and untreated HepG2 cells.
Results:
Curcumae Rhizoma, Loranthi Ramulus, and Orostachys Herba reduced mRNA expression level and protein secretion level of angiogenic factors, but Artermisiae Capillaris Herba increased it, especially VEGF and bFGF in HepG2 cells.
Conclusions:
The results indicate that Artermisiae Capillaris Herbapromotes expression of angiogenic factors but Curcumae Rhizoma, Loranthi Ramulus, and Orostachys Herba inhibit expression of angiogenic factors in HepG2 cells. The result is expected that Curcumae Rhizoma, Loranthi Ramulus, Orostachys Herba have an inhibitive effect of angiogenesis in HCC. |
| Key words:
HepG2 cell, angiogenic factor, VEGF, bFGF, TGF-β1 |
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