Single Dose Toxicity Test of <i>Mahwangyounpae-tang</i> Extract in Male SD Rats

Jeon, Kwi-ok; Cho, Dong-hee; Park, Mee-yeon; Choi, Hae-yun; Kim, Jong-dae

The Journal of Internal Korean Medicine > Volume 27(1); 2006 > Article

Original Article

The Journal of Internal Korean Medicine 2006;27(1): 102-113.

麻黃潤肺湯 추출물의 수컷 SD Rats에서 경구 단회투여 독성 평가

전귀옥, 조동희, 박미연, 최해윤, 김종대

대구한의대학교 한의과대학 내과학교실

Single Dose Toxicity Test of Mahwangyounpae-tang Extract in Male SD Rats

Kwi-ok Jeon, Dong-hee Cho, Mee-yeon Park, Hae-yun Choi, Jong-dae Kim

Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University

Correspondence

Kwi-ok Jeon ,Tel: 053-770-2081, Fax: 053-770-2169, Email: 2079272@hanmail.net

Received: February 21, 2006, Accepted: February 25, 2006, Published online: March 30, 2006.

ABSTRACT

Objectives & Methods :
To obtain the 50% lethal dose(LD50), approximated lethal dose(ALD) and approximated target organs of “Mahwangyounpae-tang” for further study into such things as repeated dose toxicity, genotoxicity and reproductive toxicity, single dose toxicity was tested in male SD rats according to KFDA Guideline 1999-61[KFDA, 1999] at dosage levels of 2,000, 1,000, 500, 250 and 125 ㎎/㎏/10㎖. In this study, mortalities, clinical signs, body weight changes and body weight gains, gross findings and weight of principal organs were detected during and/or after 14 days of single dosing.

Results & Conclusions :
After 2 or 3 days of dosing, 1 or 2 animals in 2,000 ㎎/㎏-dosing groups died. Excitation and leaping response were observed as test article-treatment related clinical signs. These abnormal signs were restricted to 2,000 and 1,000 ㎎/㎏-dosing groups and survivors recovered to normal within 3 or 4 days after dosing. Significant decrease in body weight were observed in some periods of observation in 2,000 and 1,000 ㎎/㎏-dosing group, from 1 days after dosing compared to those of vehicle control group. Significantly diminished body weight gains were observed in observation periods in 2,000 and 1,000 ㎎/㎏-dosing group compared to those of vehicle control group. Hypertrophy and hemorrhage of heart and decoloration of kidney were observed as test article-treatment related gross findings. These abnormal findings were restricted to 2,000 and 1,000 ㎎/㎏-dosing groups. A significant increase of absolute and relative heart and kidney weight were demonstrated in 2,000 ㎎/㎏-dosing groups. The value for LD50 found in this study was 2,218.57 ㎎/㎏. ALD in this study was 2,000 ㎎/㎏, and the target organs are considered to be the heart and the kidney.

Key words: Single Dose Toxicity, Mahwangyounpae-tang, SD Rats