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The Journal of Internal Korean Medicine 2006;27(1): 40-54. |
| 加味淸上補下湯의 물과 에탄올 추출법에 따른 抗알레르기 效果에 對한 硏究 |
| 허태석1, 고은정2, 정희재1, 이형구1, 배현수2, 정승기1 |
1경희대학교 한의과대학 폐계내과학 교실
2경희대학교 한의과대학 생리학교실 |
| Studying of the Effects of Gamichungsangboha-tang water-extract and ethanol-extract on IFN-γ, IL-4, T-bet, GATA-3, c-maf in Asthma Model |
| Tae-seok Heo1, Eun-jung Ko2, Hee-jae Jung1, Hyung-koo Rhee1, Hyun-su Bae2, Sung-ki Jung1 |
1Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung Hee University, Seoul, Korea
2Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, Korea |
| Correspondence |
Sung-ki Jung ,Tel: 02-958-9147, Fax: 02-958-9148, Email: jskes@unitel.co.kr
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Received: February 15, 2006, Accepted: February 24, 2006, Published online: March 30, 2006. |
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| ABSTRACT |
Objective :
This study aimed to identify the different effects of GMCSBHT water-extract and ethanol-extract on Th1/Th2 differentiation by monitoring Th1/Th2 specific cytokine secretion patterns and the transcriptional activities of T-bet, GATA-3, c-maf, INF-γ and IL-4.
Materials and Methods :
Spleen cells from eight week-old BALB/c mice were cultured in GMCSBHT extracts containing medium without activation for 24 hours and with activation for 48 hours. CD4+ T cells were isolated and mRNA expression levels of INF-γ, IL-4, T-bet, GATA-3, c-maf by RT-PCR and secretion cytokines levels of INF-γ, IL-4 by ELISA were analyzed.
Results:
GMCSBHT extracts didn't have mitogenic effects on the unstimulated CD4+ T cells. In Th1 skewed condition, GMCSBAHT water extract had no significant effects on mRNA expression levels of INF-γ, T-bet and c-maf, but inhibited mRNA expression levels of IL-4, GATA-3. It showed significantly increased secretion cytokine levels of INF-γ, but had no significant effect on secretion cytokine levels of IL-4. In Th2 skewed condition, GMCSBHT ethanol extract inhibited mRNA expression levels of INF-γ, IL-4, GATA-3 and c-maf significantly, but had no significant effects on mRNA expression levels of T-bet. It had no significant effects on secretion cytokine levels of INF-γ, but showed remarkable inhibitory effects on secretion cytokine levels of IL-4.
Conclusion:
Results suggest that on Th1/Th2 deviation, GMCSBHT water extract has both amplifying effects on Th1 differentiation and inhibitory effects on Th2, but GMCSBHT ethanol extract has stronger inhibitory effects on Th2 differentiation than on Th1. |
| Key words:
Gamichoungsangboha-tang(Jiaweiqingshangbuxia-tang), asthma. water-extract/ethanol extract, INF-γ, IL-4, T-bet, GATA-3, c-maf |
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