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The Journal of Internal Korean Medicine > Volume 27(3); 2006 > Article
The Journal of Internal Korean Medicine 2006;27(3): 725-736.
Human Neuroblastoma Cell Line BE2에 대한 健脾補腎抗癌湯의 세포고사 기전 연구
조영기1, 문미현1, 이성균1, 정현애2, 이정섭1, 남상규1, 문구1, 신선호1, 김동웅3
1원광대학교 한의과대학 전주한방병원
2원광대학교 한의과대학 익산한방병원
3서울 광동한방병원
Study of The Apoptotic Mechanisms of Gunbibosinhangam-tang on Human Neuroblastoma Cell Line BE2
Young-Kee Cho1, Mi-Hyun Moon1, Seong-Kyun Lee1, Hyun-Ae Jeong2, Jung-Sub Lee1, Sang-Kyu Nam1, Goo Moon1, Sun-Ho Shin1, Dong-Woung Kim3
1Wonkwang University Jeonju Oriental Medicine Hospital, Jeonju, Korea
2Wonkwang University Iksan Oriental Medicine Hospital, Iksan, Korea
3Kwangdong Oriental Medicine Hospital, Seoul, Korea
Correspondence  Young-Kee Cho ,Tel: 063) 270-1034, Email: youngkee77@hanmail.net
  Published online: September 30, 2006.
ABSTRACT
Objective:
In order to investigate cell death mechanisms by Gunbibosinhangam-Tang(G.B.H) in cancer cells, the activities of apoptosis signaling pathway were tested in human neuroblastoma cell line BE2.

Methods:
Viability of BE2 cells was markedly decreased by treatment of the water extract of G.B.H in a dose-dependent manner. G.B.H-induced cell death was confirmed as apoptosis characterized by chromatin condensation. We tested whether the water extract of G.B.H affects the anti-apoptotic proteins such as Bcl-XL.

Results:
Bcl-XL was uneffected by the addition of the water extract of G.B.H. in a time-dependent manner. Cleavage of PARP(poly-ADP-ribose polymerase) by activation of caspase-8 protease was also observed in BE2 cells by the treatment of the water extract of G.B.H.

Conclusion:
These results suggest that the water extract of G.B.H exerts anti-cancer effects on human neuroblastoma BE2 cells by inducing the apoptotic death via activation of intrinsic caspase cascades.
Key words: apoptosis, neuroblastoma, Gunbibosinhangam-Tang
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