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The Journal of Internal Korean Medicine 2006;27(3): 688-697. |
葫蘆巴추출물의 Peroxynitrite 제거 및 염증 인자 단백질 억제 효과 |
이철호, 정지천 |
동국대학교 한의과대학 내과학교실 |
Effect of Trigonellae Semen on Peroxynitrite Scavenging Activity and Protein Expression of Proinflammatory Mediators |
Cheol-Ho Lee, Ji-Cheon Jeong |
Deptartment of Internal Medicine, College of Oriental Medicine, Dongguk University |
Correspondence |
Ji-Cheon Jeong ,Tel: 054)770-1265, Fax: 054-770-1500, Email: jjcjh@paran.com
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Published online: September 30, 2006. |
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ABSTRACT |
Objectives: Peroxynitrite (ONOO-), ․O2 - and nitric oxide (NO) are cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer's disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate ONOO- scavenging activities, and that of its precursors, NO and ․O2 - of Trigonellae Semen.
Methods : To investigate ONOO-, NO, ․O2 - scavenging activities, fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Protein expression levels of iNOS, COX-2, NF-κB, and VCAM-1 were assayed by western blot.
Results: Trigonellae Semen markedly scavenged authentic ONOO-, ․O2 - and NO. It also inhibited ONOO- induced by ․ O2 - and NO which are derived from SIN-1. Furthermore, Trigonellae Semen inhibited ONOO-, ․O2 - and NO generation in LPS-treated ICR mouse kidney postmitochondria. Trigonellae Semen inhibited gene expression of iNOS, COX-2, VCAM-1 and NF-κB (p65) activation.
Conclusions: These results suggest that Trigonellae Semen is an effective ONOO-, ․O2 - and NO scavenger, and that this substance has a potential role as an inhibitor of the aging process, and in therapy against age-related diseases. |
Key words:
Trigonellae Semen, peroxynitrite, nitric oxide, reactive oxygen species, iNOS, COX-2, VCAM-1, NF-κB |
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