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The Journal of Internal Korean Medicine > Volume 27(3); 2006 > Article
The Journal of Internal Korean Medicine 2006;27(3): 688-697.
葫蘆巴추출물의 Peroxynitrite 제거 및 염증 인자 단백질 억제 효과
이철호, 정지천
동국대학교 한의과대학 내과학교실
Effect of Trigonellae Semen on Peroxynitrite Scavenging Activity and Protein Expression of Proinflammatory Mediators
Cheol-Ho Lee, Ji-Cheon Jeong
Deptartment of Internal Medicine, College of Oriental Medicine, Dongguk University
Correspondence  Ji-Cheon Jeong ,Tel: 054)770-1265, Fax: 054-770-1500, Email: jjcjh@paran.com
  Published online: September 30, 2006.
Peroxynitrite (ONOO-), ․O2 - and nitric oxide (NO) are cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer's disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate ONOO- scavenging activities, and that of its precursors, NO and ․O2 - of Trigonellae Semen.

Methods :
To investigate ONOO-, NO, ․O2 - scavenging activities, fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Protein expression levels of iNOS, COX-2, NF-κB, and VCAM-1 were assayed by western blot.

Trigonellae Semen markedly scavenged authentic ONOO-, ․O2 - and NO. It also inhibited ONOO- induced by ․ O2 - and NO which are derived from SIN-1. Furthermore, Trigonellae Semen inhibited ONOO-, ․O2 - and NO generation in LPS-treated ICR mouse kidney postmitochondria. Trigonellae Semen inhibited gene expression of iNOS, COX-2, VCAM-1 and NF-κB (p65) activation.

These results suggest that Trigonellae Semen is an effective ONOO-, ․O2 - and NO scavenger, and that this substance has a potential role as an inhibitor of the aging process, and in therapy against age-related diseases.
Key words: Trigonellae Semen, peroxynitrite, nitric oxide, reactive oxygen species, iNOS, COX-2, VCAM-1, NF-κB
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