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The Journal of Internal Korean Medicine > Volume 40(3); 2019 > Article
The Journal of Internal Korean Medicine 2019;40(3): 356-368.
doi: https://doi.org/10.22246/jikm.2019.40.3.356
흑축이 3T3-L1 세포와 비만 유도 흰 쥐에 미치는 영향
신만호1, 이다은2, 장혜연2, 이영수2
1동신대학교 대학원
2동신대학교 광주한방병원 한방내과
Effects of Pharbitidis Semen on 3T3-L1 Cells and Obesity-induced Rats
Man-ho Shin1, Da-eun Lee2, Hye-yeon Jang2, Young-su Lee2
1Dong-Shin University Graduate School
2Dept. of Oriental Internal Medicine, Dong-Shin University Gwangju Oriental Hospital
Correspondence  Young-su Lee ,Tel: 062-350-7203, Fax: 062-350-7141, Email: hefari92@hanmail.net
Received: April 29, 2019,   Accepted: July 13, 2019,   Published online: June 30, 2019.
ABSTRACT
Objectives:
This study was designed to evaluate the efficacy of Pharbitidis Semen (PS) on obesity using 3T3-L1 cells and high-fat-diet rats.

Methods:
After the administration of PS at a concentration of 0.125, 0.25, and 0.50 mg/ml in 3T3-L1 cells, the degree of differentiation of adipocytes was measured by Oil Red O staining. The experimental animals were divided into five groups in vivo. Normal group: diet-fed normal group (Normal), Control group (Control): high-fat-diet (HFD)-fed, Positive control group (PC): HFD+Contrave 200 mg/kg, PS_Low group: HFD+PS 250 mg/kg group, PS_High group: HFD+PS 500 mg/kg.

Results:
The total cholesterol, low density lipoprotein-cholesterol (LDL-cholesterol), and triglycerides (TG) content of the PS medication groups were significantly lower than those of the control group. In addition, high density lipoprotein cholesterol (HDL-cholesterol) tended to increase, and free fatty acid, total lipid, and phospholipid tended to decrease, but the decrease was not statistically significant. The administration of PS inhibited the differentiation of 3T3-L1 adipocytes, and a statistically significant difference was found in the group administered with 0.125 mg/ml. The mRNA expression of PPAR-γ and leptin, which are involved in the differentiation of adipocytes, was observed using reverse transcription polymerase chain reaction (RT-PCR). The expressions of PPAR-γ and leptin significantly decreased in comparison with the control and the positive control.

Conclusions:
PS can be applied clinically against obesity.
Key words: Pharbitidis Semen, 3T3-L1 cell, leptin, adiponectin, PPAR-γ
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