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The Journal of Internal Korean Medicine > Volume 40(1); 2019 > Article
The Journal of Internal Korean Medicine 2019;40(1): 58-69.
doi: https://doi.org/10.22246/jikm.2019.40.1.58
한수대보원이 포유동물인 생쥐 모델에서 골 손실 및 RANKL 유도 파골세포 분화에 미치는 영향
장시성1, 류홍선2, 전찬용3, 황귀서1
1가천대학교 한의과대학
2한의본가 연구소
3가천대학교 부속길한방병원 내과학교실
Effects of Hansu-Daebowon (HDW) on RANKL-induced Osteoclast Differentiation and Bone Loss in Mammal Model
Si-sung Jang1, Hong-sun Ryu2, Chan-yong Jeon3, Gwi-seo Hwang1
1Gachon University College of Korean Medicine
2Hanibonga Research Center
3Dept. of Korean Internal Medicine, Gachon University Gil Oriental Medical Hospital
Correspondence  Gwi-seo Hwang ,Tel: 031-750-5421, Email: seoul@gachon.ac.kr
Received: February 21, 2019,   Accepted: March 29, 2019,   Published online: March 30, 2019.
ABSTRACT
Objective:
This study investigated the effects of Hansu-Daebowon (HDW) on bone resorption in vitro and bone loss in vivo.

Methods:
Osteoclast differentiation was measured by counting TRAP (+) MNC formed from RAW 264.7 in the presence of RANKL. Bone pit formation was determined in an artificial bone slice loaded with RANKL-stimulated osteoclasts. To elucidate the mechanisms of the inhibitory effects of HDW on bone resorption and osteoclast differentiation, osteoclastogenic genes (i.e. TRAP, MMP-9, NFATc1, c-Fos, and Cathepsin K) were measured using real time PCR. Furthermore, bone loss was observed using micro-CT in an LPS-treated mammal model.

Results:
HDW inhibited the bone pit formation in vitro and inhibited bone loss in vivo. Moreover, HDW decreased the number of TRAP (+) MNCs in the presence of RANKL, and HDW inhibited the expressions of cathepsin K, MMP-9, TRAP, NFATc1, and c-Fos in the osteoclasts.

Conclusion:
HDW exerts inhibitory effects on bone loss and bone resorption resulting from the inhibitions of osteoclast differentiation and osteoclastogenic gene expression.
Key words: Hansu-Daebowon (HDW), osteoclast differentiation, TRAP, cathepsin K, NFATc1, bone loss, bone resorption
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